Effect of cell cycle synchronization on cadmium-induced apoptosis and necrosis in NRK-52E cells
| Autor: | Yan Yuan, Wenxuan Dong, Jianchun Bian, Tongwang Luo, Zhonggui Gong, Zongping Liu, Jianhong Gu, Yun Tan, Wenjing Liu, Hui Zou, Chunyan Shao, Qi Yu, Jiaqiao Zhu |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
inorganic chemicals Necrosis chemistry.chemical_element Apoptosis Biology Cell Line 03 medical and health sciences 0302 clinical medicine medicine Animals Cell synchronization Molecular Biology Cadmium Cell Cycle Cell Biology biochemical phenomena metabolism and nutrition Metal pollution Cell biology Rats 030104 developmental biology Kidney Tubules chemistry 030220 oncology & carcinogenesis Cadmium pollution bacteria medicine.symptom Developmental Biology Research Paper |
| Zdroj: | Cell Cycle |
| Popis: | Heavy metal pollution is a problem that cannot be ignored. Due to the prevalence of cadmium in the environment and its harmful effects on humans, cadmium pollution has become a research hotspot recently. The mechanism of cadmium-induced toxicity has also drawn much attention and most studies have been conducted using whole cells, but the toxicological mechanism of cadmium remains unclear. In this study, we aimed to obtain NRK-52E cells at different growth stages by various methods and analyze the differences in cadmium toxicity. The results show that the cadmium sensitivity of cells in each phase was different and the late apoptotic rate was increased significantly after 5 µM Cd treatment. In addition, cadmium easily induces apoptosis of G0- and S-phase cells, as well as necrosis of S- and M-phase cells, but has no significant effect on G1-phase cells. Overall, we first explored the differences in the effects of cadmium on NRK-52E cells at various growth phases. Besides, the findings of this study might provide a theoretical basis for further exploration of the toxicological mechanism of cadmium.Abbreviations Cd: cadmium; CDK: cyclin-dependent kinases; DAPI 2-(4-amidinophenyl)-1H-indole-6-carboxamidine; TBST: Tris-buffered saline with Tween-20; PI: propidium iodide; DMEM: Dulbecco’s Modified Eagle Medium; BCA: bicinchoninic acid |
| Databáze: | OpenAIRE |
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