cDNA microarray analysis of human keratinocytes cells of patients submitted to chemoradiotherapy and oral photobiomodulation therapy: pilot study
| Autor: | Daniel Herchenhorn, Patricia Savio de Araujo-Souza, Fabio Passetti, Carlos M.M. Araújo, Gabriel Wajnberg, Fernando Luiz Dias, Héliton Spíndola Antunes, Marcos B. Pinho, Natasha Andressa Nogueira Jorge, Joyce Luana Melo de Moraes, Claudio Gustavo Stefanoff, Mariana P. Rampini, Celia Maria Pais Viégas, Carlos Gil Ferreira |
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| Rok vydání: | 2017 |
| Předmět: |
Keratinocytes
Male 0301 basic medicine DNA Complementary Microarray Pilot Projects Dermatology 03 medical and health sciences 0302 clinical medicine Double-Blind Method Complementary DNA Gene expression medicine Mucositis Humans Low-Level Light Therapy Oral mucosa Stomatitis Microarray analysis techniques business.industry Gene Expression Profiling Mouth Mucosa Chemoradiotherapy Middle Aged Microarray Analysis medicine.disease Head and neck squamous-cell carcinoma 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation 030220 oncology & carcinogenesis Immunology Cancer research Female Surgery business |
| Zdroj: | Lasers in Medical Science. 33:11-18 |
| ISSN: | 1435-604X 0268-8921 |
| Popis: | Oral mucositis is an acute toxicity that occurs in patients submitted to chemoradiotherapy to treat head and neck squamous cell carcinoma. In this study, we evaluated differences in gene expression in the keratinocytes of the oral mucosa of patients treated with photobiomodulation therapy and tried to associate the molecular mechanisms with clinical findings. From June 2009 to December 2010, 27 patients were included in a randomized double-blind pilot study. Buccal smears from 13 patients were obtained at days 1 and 10 of chemoradiotherapy, and overall gene expression of samples from both dates were analyzed by complementary DNA (cDNA) microarray. In addition, samples from other 14 patients were also collected at D1 and D10 of chemoradiotherapy for subsequent validation of cDNA microarray findings by qPCR. The expression array analysis identified 105 upregulated and 60 downregulated genes in our post-treatment samples when compared with controls. Among the upregulated genes with the highest fold change, it was interesting to observe the presence of genes related to keratinocyte differentiation. Among downregulated genes were observed genes related to cytotoxicity and immune response. The results indicate that genes known to be induced during differentiation of human epidermal keratinocytes were upregulated while genes associated with cytotoxicity and immune response were downregulated in the laser group. These results support previous clinical findings indicating that the lower incidence of oral mucositis associated with photobiomodulation therapy might be correlated to the activation of genes involved in keratinocyte differentiation. |
| Databáze: | OpenAIRE |
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