The origin of deletion 22q11 in chronic lymphocytic leukemia is related to the rearrangement of immunoglobulin lambda light chain locus
| Autor: | Michael Doubek, Katerina Stano Kozubik, Jiri Mayer, Šárka Pospíšilová, Marek Borsky, Boris Tichy, Yvona Brychtová, Katerina Musilova, Marek Mráz, Karla Plevová, Petr Kuglík |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Cancer Research Chromosomes Human Pair 22 Chronic lymphocytic leukemia Locus (genetics) In situ hybridization Biology Polymerase Chain Reaction law.invention 03 medical and health sciences 0302 clinical medicine Immunoglobulin lambda-Chains Antigens Neoplasm law medicine Humans Gene In Situ Hybridization Fluorescence Polymerase chain reaction Aged 030304 developmental biology Aged 80 and over Gene Rearrangement Genetics Comparative Genomic Hybridization 0303 health sciences Hematology Gene rearrangement Middle Aged Prognosis medicine.disease Leukemia Lymphocytic Chronic B-Cell Molecular biology Leukemia Oncology 030220 oncology & carcinogenesis Female Chromosome Deletion Follow-Up Studies Comparative genomic hybridization |
| Zdroj: | Leukemia Research Leukemia Research; Vol 37 |
| ISSN: | 0145-2126 |
| Popis: | The technology of array comparative genomic hybridization (array-CGH/aCGH) enabled the identification of novel genomic aberrations in chronic lymphocytic leukemia (CLL) including the monoallelic and biallelic deletions affecting 22q11 locus. In contrast to previous publications, we hypothesized that the described 22q11 deletions are a consequence of the rearrangement of immunoglobulin lambda light chain locus (IGL) segments surrounding several protein-coding genes located in this region. Indeed, using array-CGH and PCR analysis we show that all deletions (n=7) affecting the 22q11 locus in our cohort (n=40) are based on the physiological mechanism of IGL rearrangement. This demonstrates that this loss of genetic material is likely not pathogenic and in fact is merely a marker of IGL rearrangement. |
| Databáze: | OpenAIRE |
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