Isolation and phylogenetic analysis of porcine deltacoronavirus from pigs with diarrhoea in Hebei province, China
| Autor: | W.-Y. Gu, Lin-Shan Hou, Yuzhu Zuo, Jinghui Fan, S.-X. Luo, Qian-Kai Shi, F. Zhong, B.-J. Liu |
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| Rok vydání: | 2018 |
| Předmět: |
Diarrhea
0301 basic medicine China Hebei province Genes Viral Swine Biology medicine.disease_cause Evolution Molecular Feces 03 medical and health sciences Enteric disease Genetic Evolution medicine Animals Phylogeny Coronavirus Swine Diseases General Veterinary General Immunology and Microbiology Phylogenetic tree phylogenetic analysis Genomics Original Articles General Medicine medicine.disease Isolation (microbiology) Genus Deltacoronavirus Virology Intestines Porcine deltacoronavirus 030104 developmental biology Coinfection Original Article Coronavirus Infections isolation porcine deltacoronavirus |
| Zdroj: | Transboundary and Emerging Diseases |
| ISSN: | 1865-1674 |
| DOI: | 10.1111/tbed.12821 |
| Popis: | Summary Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus in the genus Deltacoronavirus that can cause enteric disease with clinical signs including diarrhoea, vomiting, dehydration and mortality in neonatal piglets. Although evidence of the prevalence of PDCoV in China is accumulating, little published information about Chinese PDCoV isolates is available. In this study, we investigated the presence of PDCoV in 49 faecal/intestinal samples from piglets with diarrhoea on different farms in Hebei province. Five samples (10.2%) were positive for PDCoV, but no coinfection of PDCoV with other enteropathogens was observed. A PDCoV strain named HB‐BD was successfully isolated from the intestinal contents of a diarrhoeic piglet and serially propagated in swine testicular (ST) cells for >40 passages. The complete genome of the HB‐BD strain was sequenced and analysed. Genomic analysis showed that the HB‐BD strain had a closer relationship with Chinese strains than those from other countries and was grouped within the Chinese PDCoV cluster. The results of this study will be valuable for further research of PDCoV genetic evolution and development of effective diagnostic reagents, assays and potential vaccines against newly emerged PDCoV strains. |
| Databáze: | OpenAIRE |
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