Detailed Molecular Delineation of 13q14.3 Loss in B-Cell Chronic Lymphocytic Leukemia
| Autor: | Yie Liu, R. Chapman, David Oscier, Mats Merup, Arati Iyengar, Gösta Gahrton, Dan Grandér, Gunnar Juliusson, Rachel E. Ibbotson, Stefan Einhorn, Ganka Ivanova, Anne Gardiner, Vadim Brodyansky, Eugene R. Zabarovsky, Mary Tiller, N. K. Yankovsky, Xiushan Wu, Omid Rasool, Martin Corcoran |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Genetic Markers
Genetics Candidate gene Chromosomes Human Pair 13 Contig Genome Human Immunology Chromosome Locus (genetics) Cell Biology Hematology Biology Leukemia Lymphocytic Chronic B-Cell Retinoblastoma Protein Biochemistry Molecular biology Loss of heterozygosity Cosmid Humans Deletion mapping Chromosomes Artificial Yeast Sequence Deletion Southern blot |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v91.4.1382 |
| Popis: | A region of chromosome 13q14.3, telomeric to the Retinoblastoma gene RB-1 is frequently deleted in patients with B-cell chronic lymphocytic leukemia (B-CLL). A cosmid and P1-derived artificial chromosome (PAC) contig spanning over 600 kb has been constructed, which encompasses this locus. The contig clones have been used to order a number of markers along the minimally deleted region and to localize a series of CpG islands corresponding to possible candidate genes. A novel polymorphic dinucleotide repeat, 6E3.2, present in one of the ordered cosmid clones has been isolated for use in deletion mapping studies of patient DNA. Leukemic samples from 229 CLL patients have been screened for loss of heterozygosity using microsatellite markers and analyzed for hemizygous and homozygous deletions by Southern blot techniques using genomic probes selected from cosmids across the region. Hemizygous deletions were found in 31% of cases with an additional 10% showing homozygous loss. The use of these probes has defined the commonly deleted area to less than 130 kb, centromeric to the locus D13S272. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|