Impairment of maturation of BMP-6 (35 kDa) correlates with delayed fracture healing in experimental diabetes
| Autor: | Rami Abboud, Weijie Wang, Qidong Guo, Zheng Guo |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty lcsh:Diseases of the musculoskeletal system Bone Morphogenetic Protein 6 Healing Nonunion Delayed union Bone healing Diabetes Mellitus Experimental Rats Sprague-Dawley BMP-6 03 medical and health sciences 0302 clinical medicine lcsh:Orthopedic surgery Downregulation and upregulation Diabetes mellitus Internal medicine Animals Medicine Orthopedics and Sports Medicine Fracture Healing 030222 orthopedics Femur fracture business.industry Diabetes medicine.disease Rats lcsh:RD701-811 Fracture 030104 developmental biology Endocrinology Callus Orthopedic surgery Surgery lcsh:RC925-935 business Femoral Fractures Research Article Calcification |
| Zdroj: | Journal of Orthopaedic Surgery and Research Journal of Orthopaedic Surgery and Research, Vol 15, Iss 1, Pp 1-11 (2020) |
| ISSN: | 1749-799X |
| DOI: | 10.1186/s13018-020-01705-7 |
| Popis: | BackgroundAlthough it is known that diabetes interferes with fracture healing, the mechanisms remain poorly understood. The aim of this study was to investigate the correlation of BMP-6 and BMP-9 with the impairment in fracture healing in diabetes, by analyses of the difference in size and calcification of the callus, mechanical endurance, and expressing BMP-6 and BMP-9 in the callus, using a clinical related diabetic rodent model.MethodsWe evaluated femur fracture healing by quantification of size and calcification of the callus by X-ray, histological and histochemical images, loading capacity of the fractured bone, and amount of BMP-6 in the callus and the bones using Western blot assay.ResultsSignificant upregulation of BMP-6 in the callus and the fractured bones of both non-diabetic and the diabetic animals was observed, at the end of the second and the fourth weeks after fracture. However, significantly lower levels of BMP-6 at 35 kDa with smaller sizes of calcified callus and poor loading capacity of the healing bones were detected in the diabetic animals, compared to the non-diabetic controls. The impairment of the maturation procedure of BMP-6 (35 kDa) from precursors may be underlying the downregulation of the BMP-6 in diabetic animals.ConclusionsIt could be concluded that the delayed fracture healing in the diabetic animals is correlated with deficiency of BMP-6 (35 kDa), which may be caused by impairment of maturation procedure of BMP-6 from precursors to functioning format. This is a primary study but an important step to explore the molecular pathogenesis of impairment of fracture healing in diabetes and to molecular therapeutic approach for the impairment of fracture healing. |
| Databáze: | OpenAIRE |
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