Induced CD10 expression during monocyte-to-macrophage differentiation identifies a unique subset of macrophages in pancreatic ductal adenocarcinoma
| Autor: | Guohe Lin, Jun Wang, Yunda Song, Shengping Li, Kaili Xing, Shuxin Sun, Lianghe Lu, Yize Mao, Xin Hua, Chaobin He, Xin Huang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
CD14 Biophysics Biology Immunofluorescence Biochemistry Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Immune system immune system diseases Cell Line Tumor hemic and lymphatic diseases medicine Humans Molecular Biology Cells Cultured medicine.diagnostic_test Macrophages Monocyte Cell Differentiation Cell Biology Prognosis medicine.disease Gene Expression Regulation Neoplastic Pancreatic Neoplasms 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Cancer research Phorbol Immunohistochemistry Neprilysin Infiltration (medical) Carcinoma Pancreatic Ductal |
| Zdroj: | Biochemical and Biophysical Research Communications. 524:1064-1071 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2020.02.042 |
| Popis: | Objective Tumor associated macrophages (TAMs) promoted pancreatic ductal adenocarcinoma (PDAC) initiation and progression. In this study we aimed to evaluate CD10 expression by monocytes/macrophages and its clinical significance in PDAC. Methods Human CD14+ peripheral blood monocytes were isolated and cultured for 6–7 days to differentiate into macrophages in vitro. Monocytic THP-1 cells were cultured and treated with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) for 72 h to induce macrophage differentiation. Reverse transcription-quantitative PCR, immunohistochemistry, immunofluorescence, multiplex immunohistochemical staining and flow cytometry were performed to detect CD10 expression. In addition, the correlations between CD10 expression and immune cells infiltration were investigated through TIMER or GEPIA. Finally, Kaplan-Meier plotter and GEPIA databases were adopted to evaluate the influence of CD10 on clinical prognosis. Results Our results indicated that CD10 was expressed by a subset of human monocytes and many more cells expressed CD10 after differentiation into macrophages in vitro (13.19% vs. 41.39%; P Conclusions In this study we demonstrated that CD10 was expressed by human primary monocytes, human monocyte-derived macrophages and TAMs, and was correlated with poor prognosis in PDAC, suggesting CD10 to be a potential therapeutic target in PDAC. |
| Databáze: | OpenAIRE |
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