Evaluation of mouse embryos produced in vitro after electromagnetic waves exposure; Morphometric study
| Autor: | Reza Ahmadi, Ayoob Rostamzadeh, Afshin Nazari, Maryam Anjomshoa, Mohsen Mohammadi, Omar Ghaderi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
030103 biophysics Pathology medicine.medical_specialty medicine.medical_treatment Perivitelline space embryo morphometry Andrology 03 medical and health sciences Statistical significance medicine Irradiation fertility lcsh:R5-920 Pregnancy In vitro fertilisation medicine.diagnostic_test business.industry MRI (Magnetic resonance imaging) Magnetic resonance imaging Embryo medicine.disease In vitro Original Article lcsh:Medicine (General) business |
| Zdroj: | Electronic Physician, Vol 8, Iss 1, Pp 1701-1710 (2016) Electronic Physician |
| ISSN: | 2008-5842 |
| DOI: | 10.19082/1701 |
| Popis: | INTRODUCTION Today, the use of electromagnetic waves in medical diagnostic devices, such as magnetic resonance imaging (MRI), has increased, and many of its biological effects have been reported. The aim of the present study was to assess the biological effects of 1.5 Tesla (T) magnetic resonance imaging (MRI) on fertility and reproductive parameters. METHODS Eighty adult male and female NMRI mice (NMRI: Naval Medical Research Institute) of age 6-8 weeks were studied and randomly divided into two study and control groups. After confirmation of pregnancy, the mice in the study group were exposed to the MRI (1.5 T) machine's waves over the next three weeks, once a week for 36 minutes. One day and thirty-five days after the last radiation, the mice were killed in order to do the in vitro fertilization (IVF) by neck beads' displacement and the impact on the evolution of embryos, and its quality was studied. Data were analyzed using SPSS version 20 and the significance level of less than 0.05 was considered. RESULTS Embryo morphometry showed that the total diameter and the cytoplasm diameter of the study group embryos suffered significant reduction compared to the control group, 1 day after the last irradiation (p < 0.05), but the diameter of the perivitelline space of this group's embryos had a significant increase (p < 0.05). The qualitative results during 35 days after irradiation showed that morphologically parameters of the embryos in the study group had no significant differences from the control group. CONCLUSION Exposure to MRI irradiation can transiently disturb the development of mouse embryos and fertility, but these effects are reversible 35 days after the last irradiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|