A simple LC-MS/MS method for the determination of cortisol, cortisone and tetrahydro-metabolites in human urine: assay development, validation and application in depression patients
| Autor: | Yong-ning Lu, Chaoran Zhu, Fen Chen, Xue-jia Zhai |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Cholestenone 5 alpha-Reductase Hydrocortisone Clinical Biochemistry Pharmaceutical Science Urine Reductase Analytical Chemistry chemistry.chemical_compound Young Adult Tandem Mass Spectrometry Internal medicine 11-beta-Hydroxysteroid Dehydrogenase Type 2 Drug Discovery 11-beta-Hydroxysteroid Dehydrogenase Type 1 medicine Humans Chronic stress Tetrahydrocortisone Glucocorticoids Tetrahydrocortisol Spectroscopy Depression (differential diagnoses) chemistry.chemical_classification Depressive Disorder Major Chromatography Chemistry Middle Aged Cortisone Enzyme Endocrinology Case-Control Studies medicine.drug Chromatography Liquid |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 107 |
| ISSN: | 1873-264X |
| Popis: | Chronic stress as well as major depressive disorders is associated with cortisol metabolism. Two enzymes modulate cortisol (F) and cortisone (E) interconversion: 11β-hydroxysteroid dehydrogenase type 1 and type 2 (11β-HSD1 and 11β-HSD2). Furthermore, F and E were inactivated by 5α and 5β reductases to their tetrahydro-metabolites: tetrahydrocortisol (THF), allo-tetrahydrocortisol (5α-THF) and tetrahydrocortisone (THE). To better understand depression a LC-MS/MS method for simultaneous determination of F, E THF, 5α-THF and THE in human urine has been developed and validated. The quantification range was 0.1-160 ng mL(-1) for F and E, and 0.2-160 ng mL(-1) for the tetrahydro-metabolites, with >86.1% recovery for all analytes. The nocturnal urine concentrations of F, E and tetrahydro-metabolites in 12 apparently healthy male adult volunteers and 12 drug-free male patients (age range, 20-50 years) with a diagnosis of depression were analyzed. A series of significant changes in glucocorticoid metabolism can be detected: F/E ratios and (THF+5α-THF)/THE ratios as well as F and THF concentrations were significantly higher in depression patients than in healthy subjects (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect