Comparable stx 2a expression and phage production levels between Shiga toxin‐producing Escherichia coli strains from human and bovine origin
| Autor: | Alejandra Krüger, Julia Burgán, Paula Maria Alejandra Lucchesi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Serotype Epidemiology 030231 tropical medicine 030106 microbiology Virulence STX2A PHAGE Biology medicine.disease_cause Microbiology STX2A EXPRESSION Pathogenesis 03 medical and health sciences 0302 clinical medicine STX2 Otras Ciencias Veterinarias medicine Shiga toxin-producing Escherichia coli Escherichia coli Prophage General Veterinary General Immunology and Microbiology Ciencias Veterinarias Mitomycin C Public Health Environmental and Occupational Health STEC PROPHAGE INDUCTION Infectious Diseases CIENCIAS AGRÍCOLAS |
| Zdroj: | Zoonoses and Public Health. 67:44-53 |
| ISSN: | 1863-2378 1863-1959 |
| DOI: | 10.1111/zph.12653 |
| Popis: | Shiga toxin-producing Escherichia coli (STEC) can cause diarrhea and severe diseases in humans such as hemolytic uremic syndrome. Shiga toxins (Stx) are encoded in prophages which play an important role in STEC pathogenesis. The pathogenic potential of STEC is considered correlated with the amount of Stx2 produced and, in particular, Stx2a is the subtype most frequently associated with high virulence. The aims of this study were to evaluate stx2a expression levels and Stx2a phage production by qPCR and double-agar-layer method in 29 STEC strains isolated from cattle and humans. Results were also compared to find possible associations with the serotype, origin or some genetic features. Heterogeneous levels of stx2a expression and Stx2a phage production were observed. Statistical comparisons identified a higher stx2a expression in response to mitomycin C in strains isolated from cattle than in those from humans, and a higher increase in phage production under inducing conditions in stx2a strains compared to stx2a/stx2c strains. Notably, most of the strains studied, regardless of serotype and origin, carried inducible Stx2a phages, and evidenced expression of stx2a which showed an increase along with phage production levels under induced conditions, suggesting a high virulence of the strains circulating in Argentina Fil: Burgán, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Krüger, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina Fil: Lucchesi, Paula Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina |
| Databáze: | OpenAIRE |
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