Chromosomal Localization and Genomic Characterization of the Mouse Melastatin Gene (Mlsn1)
| Autor: | Andrew W. Shyjan, Deborah L. Nagle, Jing Shao, Karen J. Moore, Barry J. Dussault, John J. Hunter, Elizabeth A. Woolf, John S. Smutko, Lisa Holmgren |
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| Rok vydání: | 1998 |
| Předmět: |
Male
DNA Complementary Molecular Sequence Data Melanoma Experimental TRPM Cation Channels Biology Polymerase Chain Reaction Mice Exon Gene mapping Gene expression Tumor Cells Cultured Genetics Animals Humans Inbreeding Amino Acid Sequence Promoter Regions Genetic Gene TRPM1 Chromosomes Human Pair 15 Base Sequence Chromosome Mapping Membrane Proteins Promoter Exons Blotting Northern Molecular biology Neoplasm Proteins Mice Inbred C57BL Blotting Southern genomic DNA Female Human genome |
| Zdroj: | Genomics. 54:116-123 |
| ISSN: | 0888-7543 |
| DOI: | 10.1006/geno.1998.5549 |
| Popis: | We recently described a novel gene, melastatin, whose expression is inversely correlated with melanoma aggressiveness. Chromosomal localization of this gene places it on mouse chromosome 7 and in the 15q13–q14 region of the human genome. Although expression patterns and chromosomal localization in the mouse are consistent with involvement of melastatin mutations in the mouse ruby-eye-2 defect, congenic analysis showed genetic segregation of the two loci. Cloning of the full-length human cDNA revealed a much larger transcript than we had previously identified, corresponding to a 1533-amino-acid protein product with homology to members of the transient receptor potential (Trp) family of calcium channels. The mouse melastatin gene contains 27 exons and spans at least 58 kb of genomic DNA. The promoter region of Mlsn1 contains four potential microphthalmia binding sites including an M box, a transcriptional regulatory element unique to genes with a restricted melanocytic expression pattern. A 1-kb Pvu II fragment from this region was capable of driving high levels of luciferase expression in B16 melanoma cells. |
| Databáze: | OpenAIRE |
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