Human papillomavirus detection and genotyping, by HC2, full-spectrum HPV and molecular beacon real-time HPV assay in an Irish colposcopy clinic
| Autor: | Tom D'Arcy, John J. O'Leary, Borbála Pal-Szenthe, Csaba Jeney, Michael J. Turner, Loretto Pilkington, Agnes Solt, Anette Kovács, Johanna Mózes, Helen Keegan, Sinead Cleary, Márta Benczik, Noel Bolger, Borbála Kaltenecker, Günther von Bünau, Jamie McInerney, Cara Martin, Sharon O’ Toole |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
medicine.medical_specialty Time Factors Genotype Genotyping Techniques Biology Sensitivity and Specificity Young Adult Molecular beacon Predictive Value of Tests Virology Cytology medicine Humans Human papillomavirus Genotyping Papillomaviridae Colposcopy Gynecology Cervical screening medicine.diagnostic_test Coinfection Papillomavirus Infections virus diseases Middle Aged female genital diseases and pregnancy complications Molecular Diagnostic Techniques Liquid-based cytology Female Ireland Colposcopy clinic |
| Zdroj: | Journal of virological methods. 201 |
| ISSN: | 1879-0984 |
| Popis: | Cervical screening programmes are moving towards HPV testing as part of the screening process and as a triage for colposcopy. Three HPV detection methods were evaluated using cervical cytology specimens from colposcopy patients. PreservCyt™ liquid based cytology specimens from 241 women attending colposcopy clinics with greater than 2 persistently abnormal smears were recruited through the Coombe Women and Infants University Hospital, Dublin. HPV DNA was detected by Hybrid Capture (HC2) for 13 high-risk HPV types, Full-Spectrum HPV (FS-HPV) for 49 high and low-risk types and Molecular Beacon Real-Time HPV assay (MBRT-HPV) for 16 high and low-risk types. HPV genotyping was performed using Linear Array HPV Assay (LA-HPV). HPV was detected in 83.3% (195/234), 91.9% (217/236) and 80.1% (169/211) of cytology specimens by HC2, FS-HPV and MBRT-HPV, HPV DNA detection assays. The sensitivity of the assays for the detection of high-risk HPV in cytology specimens that had a Cervical Intraepithelial Neoplasia Grade 2+ result by histology were, 98%, 97% and 94% for HC2, FS-HPV and MBRT-HPV assays with positive predictive values of 94.1%, 94.1% and 97.3%. The most common HPV genotypes were HPV 16, 31, 33, 58, 42, 61 and 53, and the most common high-risk HPV genotypes were HPV 16, 31, 33, 58, 18, 45, 59, 51, 56 and 39, with detection of multiple infections in 57.7% of all cases. FS-HPV and MBRT-HPV are highly sensitive and have a similarly high PPV as the HC2 assay for detection of HPV in patients with Cervical Intraepithelial Neoplasia Grade 2+ disease. HPV genotyping of women with persistent abnormalities is warranted prior to the introduction of HPV DNA testing in a colposcopy setting. |
| Databáze: | OpenAIRE |
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