Computer-assisted chromatin texture characterization of Feulgen-stained nuclei in a series of 331 transitional bladder cell carcinomas
| Autor: | Hendrik Roels, Willem Oosterlinck, Claude Schulman, R. van Velthoven, Cassio Zandona, Jean Lambert Pasteels, Robert Kiss, Michel Petein, A Zlotta, A. P. M. Van Der Meijden |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Urinary Bladder Biology Matrix (biology) Pathology and Forensic Medicine Prophase Image Processing Computer-Assisted Rosaniline Dyes medicine Humans Feulgen stain Coloring Agents Aged Neoplasm Staging Aged 80 and over Carcinoma Transitional Cell Mucous Membrane Staining and Labeling Middle Aged medicine.disease Chromatin Cell nucleus Transitional cell carcinoma medicine.anatomical_structure Urinary Bladder Neoplasms Female Nucleus Cytometry |
| Zdroj: | The Journal of Pathology. 173:235-242 |
| ISSN: | 1096-9896 0022-3417 |
| Popis: | The chromatin patterns of Feulgen-stained nuclei in a series of six specimens of normal mucosa and 331 transitional bladder carcinomas, including 293 superficial (Ta and T1) and 38 invasive (T2-T4) cases, were quantitatively described by means of eight parameters relating to densitometric, run-length distribution, and co-occurrence matrix features. The results show that the chromatin texture of the superficial lesions was markedly different from that of the invasive tumours, which exhibited a distinctly more dense and heterogeneous chromatin pattern. The data also show that the increasing level of malignancy, as revealed by the increasing clinical stage, was accompanied by an increase in the overall chromatin condensation level. Only some areas of the nucleus actually increased in density; other pale areas appeared concomitantly with these increasingly denser chromatin areas. This chromatin density increase corresponded to a marked increase in the frequency of small dense chromatin clumps; these joined together into very large dense chromatin clumps, which were distributed more and more heterogeneously in the nucleus as the clinical stage of the tumour increased. |
| Databáze: | OpenAIRE |
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