The selection of a standard STR panel for DNA profiling of the African elephant (Loxodonta africana) in Kenya

Autor:	Mercy W. Mwaniki, Francis Gakuya, Julius Kinuthia, Amos Alakonya, Shadrack Muya, Cindy Kim Harper, Charles Kimwele, Erastus Gatebe, Anne W. T. Muigai
Rok vydání:	2014
Předmět:	education.field_of_study Population Zoology Poaching Biology African elephant DNA profiling biology.animal Multiplex polymerase chain reaction Genetics Microsatellite education Genotyping Ecology Evolution Behavior and Systematics Selection (genetic algorithm)
Zdroj:	Conservation Genetics Resources. 7:305-307
ISSN:	1877-7260 1877-7252
Popis:	The African elephant (Loxodonta africana) population in Kenya is declining at an alarming rate due to habitat destruction, human-elephant conflicts and the cur- rent escalation of poaching for ivory. This study estab- lished a standard protocol for forensic analysis of L. africana and their products such as ivory. Three multiplex PCR panels consisting of 17 Short Tandem Repeat markers were selected from 40 markers using bioinformatics tools, amplification and polymorphism. Genotyping was suc- cessful and reproducible. This method is efficient, accurate and cost effective and has potential for application in conservation of L. africana.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e1e72fc0d80c4cc5c0561bfacb2a42fa https://doi.org/10.1007/s12686-014-0366-6 Zobrazit plný text záznamu Full text from SpringerLink