Regulation of cyclin E gene expression by the human papillomavirus type 16 E7 oncoprotein
| Autor: | Karin Zerfaß-Thome, Pidder Jansen-Dürr, Jürgen W. Botz, Almut Schulze, Beate Vogt, Werner Zwerschke |
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| Rok vydání: | 1999 |
| Předmět: |
Transcriptional Activation
Cyclin E Transcription Genetic Sp1 Transcription Factor Cyclin D Papillomavirus E7 Proteins Cyclin A Cell Cycle Proteins Cell Line Mice Cyclin D1 Virology Gene expression Animals Drosophila Proteins Humans E2F Promoter Regions Genetic Papillomaviridae Binding Sites biology Promoter 3T3 Cells Oncogene Proteins Viral Molecular biology E2F Transcription Factors DNA-Binding Proteins Gene Expression Regulation biology.protein Trans-Activators Drosophila Carrier Proteins Transcription Factor DP1 Cyclin A2 Retinoblastoma-Binding Protein 1 Transcription Factors |
| Zdroj: | The Journal of general virology. 80 |
| ISSN: | 0022-1317 |
| Popis: | In this study, we characterized the 5′ regulatory region of the murine cyclin E gene and analysed activation of the gene by the E7 oncogene of human papillomavirus type 16 in transfection experiments. We found that the murine cyclin E promoter is composed of multiple regulatory elements, and we present evidence for at least two independent transcription units, designated P1 and P2. Overlapping binding sites for the cellular transcription factors Sp1 and E2F were identified in both promoters, and we found that E2F-mediated activation of transcription is inhibited by Sp1 in cotransfection experiments. The E2F/Sp1 binding sites contribute to transcriptional activation by E7, and the data suggest that the cyclin E gene is rendered E7-inducible through the combination of severalcis-acting elements which display only weak intrinsic responsiveness to E7. |
| Databáze: | OpenAIRE |
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