Site-Selective Synthesis of 15N- and 13C-Enriched Flavin Mononucleotide Coenzyme Isotopologues
| Autor: | Syam Sundar Neti, C. Dale Poulter |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Magnetic Resonance Spectroscopy Pyrazine Pyrimidine Flavin Mononucleotide Stereochemistry Coenzymes Flavin mononucleotide Flavoprotein Flavin group Spectrum Analysis Raman 010402 general chemistry Photochemistry Mass spectrometry 01 natural sciences Mass Spectrometry Cofactor 03 medical and health sciences chemistry.chemical_compound Carbon Isotopes Nitrogen Isotopes biology Organic Chemistry Nuclear magnetic resonance spectroscopy 0104 chemical sciences 030104 developmental biology chemistry Isotope Labeling biology.protein |
| Zdroj: | The Journal of Organic Chemistry. 81:5087-5092 |
| ISSN: | 1520-6904 0022-3263 |
| DOI: | 10.1021/acs.joc.6b00640 |
| Popis: | Flavin mononucleotide (FMN) is a coenzyme for numerous proteins involved in key cellular and physiological processes. Isotopically labeled flavin is a powerful tool for studying the structure and mechanism of flavoenzyme-catalyzed reactions by a variety of techniques, including NMR, IR, Raman, and mass spectrometry. In this report, we describe the preparation of labeled FMN isotopologues enriched with (15)N and (13)C isotopes at various sites in the pyrazine and pyrimidine rings of the isoalloxazine core of the cofactor from readily available precursors by a five-step chemo-enzymatic synthesis. |
| Databáze: | OpenAIRE |
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