Evidence that the WNT-inducible growth arrest-specific gene 1 encodes an antagonist of sonic hedgehog signaling in the somite
| Autor: | Laura Buttitta, Chen-Ming Fan, Catherine S. Lee |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
animal structures
Cell Cycle Proteins Biology GPI-Linked Proteins Transfection Mice Genes Reporter Proto-Oncogene Proteins Notochord Chlorocebus aethiops medicine Animals Hedgehog Proteins Sonic hedgehog Body Patterning Gene Library Embryonic Induction Multidisciplinary Neural tube Wnt signaling pathway Membrane Proteins Protein-Tyrosine Kinases Zebrafish Proteins Biological Sciences Molecular biology Surface ectoderm Hedgehog signaling pathway Recombinant Proteins Wnt Proteins Somite medicine.anatomical_structure Gene Expression Regulation Somites embryonic structures COS Cells biology.protein Mutagenesis Site-Directed Trans-Activators Ectopic expression Signal Transduction |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 98(20) |
| ISSN: | 0027-8424 |
| Popis: | The dorsal–ventral polarity of the somite is controlled by antagonistic signals from the dorsal neural tube/surface ectoderm, mediated by WNTs, and from the ventral notochord, mediated by sonic hedgehog (SHH). Each factor can act over a distance greater than a somite diameter in vitro , suggesting they must limit each other's actions within their own patterning domains in vivo . We show here that the growth-arrest specific gene 1 ( Gas1 ), which is expressed in the dorsal somite, is induced by WNTs and encodes a protein that can bind to SHH. Furthermore, ectopic expression of Gas1 in presomitic cells attenuates the response of these cells to SHH in vitro . Taken together, these data suggest that GAS1 functions to reduce the availability of active SHH within the dorsal somite. |
| Databáze: | OpenAIRE |
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