Pb and Hg heavy metal tolerance of single- and mixedspecies biofilm (Rhodotorula mucilaginosa and Escherichia coli)
| Autor: | Aleksandar Ostojić, Sandra Grujić, Ljiljana R. Čomić, Ivana D. Radojević, Sava Vasić, Anica Buzejic |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
heavymetals Tetracycline Rhodotorula mucilaginosa 010501 environmental sciences medicine.disease_cause 01 natural sciences biofilm Microbiology 03 medical and health sciences Minimum inhibitory concentration chemistry.chemical_compound Bioremediation Escherichia coli medicine Crystal violet lcsh:Science 0105 earth and related environmental sciences tolerance Chromatography biology Biofilm biochemical phenomena metabolism and nutrition biology.organism_classification mixed-species 6. Clean water 030104 developmental biology chemistry lcsh:Q Bacteria medicine.drug |
| Zdroj: | Kragujevac Journal of Science (2016) (38):115-124 Kragujevac Journal of Science, Vol 2016, Iss 38, Pp 115-124 (2016) |
| ISSN: | 1450-9636 |
| Popis: | The aim of this study was to examine heavy metal tolerance (lead (Pb2+) and mercury (Hg2+)) of single- and mixed-species biofilms, formed by yeast Rhodotorula mucilaginosa and bacteria Escherichia coli LM1. Single- and mixed-species biofilms were quantified by crystal violet test and the absorbance was measured using microplate reader (OD570). The minimal inhibitory concentration (MIC) and the minimal lethal concentration (MLC) were determined and the results were confirmed by fluorescence microscopy. The significant difference in lead tolerance was observed between the mixed- and the single-species biofilms. The MIC of lead (Pb2+) for the examined biofilms (E. coli LM1, R. mucilaginosa and R. mucilaginosa / E. coli) was recorded at concentrations of 4000 μg/ml, 4000 μg/ml and 16000 μg/ml, respectively. The MIC of mercury (Hg2+) for the biofilms was noticed at concentrations of 31.25 μg/ml, 250 μg/ml and 250 μg/ml, respectively. Standard antibiotics (amphotericin B and tetracycline) were used as positive control. Results obtained for single-species biofilms were compared in between and with the results obtained for mixed-species biofilm. The tolerance of the mixed- species biofilm was higher in comparison to the singlespecies biofilms and the results were confirmed by a fluoresecence microscope. The obtained results suggest that the R. mucilaginosa / E. coli biofilm may have a potential to be used in bioremediation of wastewaters contaminated with lead and mercury. |
| Databáze: | OpenAIRE |
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