Core–shell PLA–PVA porous microparticles as carriers for bacteriocin nisin
| Autor: | Pavlina Holcapkova, Vladimir Sedlarik, Petra Stoplova, Martina Hrabalikova |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Vinyl alcohol
Materials science Scanning electron microscope Polyesters Pharmaceutical Science Bioengineering 02 engineering and technology 010402 general chemistry 01 natural sciences Glutarates chemistry.chemical_compound Colloid and Surface Chemistry Polylactic acid Zeta potential Organic chemistry Particle Size Physical and Theoretical Chemistry Microparticle Nisin Antibacterial agent Drug Carriers Organic Chemistry 021001 nanoscience & nanotechnology Listeria monocytogenes Anti-Bacterial Agents 0104 chemical sciences chemistry Chemical engineering Polyvinyl Alcohol Microscopy Electron Scanning 0210 nano-technology Porosity BET theory |
| Zdroj: | Journal of Microencapsulation. 34:243-249 |
| ISSN: | 1464-5246 0265-2048 |
| DOI: | 10.1080/02652048.2017.1324919 |
| Popis: | This work is focused on preparation of novel porous type of core-shell-structured microparticles based on polylactide (shell) and poly(vinyl alcohol) cross-linked with glutaric acid (GA) (core) prepared by water-in-oil-in-water solvent evaporation technique. The microparticle systems were used as delivery systems for immobilisation of model antibacterial agent - nisin. The effect of cross-linking and the initial amount of nisin on their morphology was investigated using scanning electron microscopy, BET analysis, zeta potential measurement and Fourier transform infra-red spectroscopy. Encapsulation efficiency and release profile of nisin from the microparticles were studied by high performance liquid chromatography. Antibacterial activity of the prepared systems was tested by dilution and spread plate technique. Results showed the microparticles in the size range of 9-16 μm in diameter with spherical multi-hollow core-shell structure. The presence of cross-linking agent GA influences the release profile of the peptide and has synergistic effect on Listeria monocytogenes growth reduction. |
| Databáze: | OpenAIRE |
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