Correlation of Redondovirus and Entamoeba gingivalis Detections in the Human Oral Cavity Suggests That This Amoeba Is Possibly the Redondovirus Host
| Autor: | Marine Makoa-Meng, Rayan Semmar, Angéline Antezack, Gwilherm Penant, Bernard La Scola, Virginie Monnet-Corti, Philippe Colson |
|---|---|
| Přispěvatelé: | Microbes évolution phylogénie et infections (MEPHI), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille), Aix-Marseille Université - Faculté d'odontologie (AMU ODONTO), Aix Marseille Université (AMU), Assistance Publique - Hôpitaux de Marseille (APHM), ANR-10-IAHU-0003,Méditerranée Infection,I.H.U. Méditerranée Infection(2010) |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
redondovirus Organic Chemistry General Medicine [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Catalysis Computer Science Applications Inorganic Chemistry Entamoeba [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology oral cavity [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology human Physical and Theoretical Chemistry periodontitis Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, 2023, 24 (7), pp.6303. ⟨10.3390/ijms24076303⟩ International Journal of Molecular Sciences; Volume 24; Issue 7; Pages: 6303 |
| ISSN: | 1661-6596 1422-0067 |
| DOI: | 10.3390/ijms24076303⟩ |
| Popis: | International audience; The virome of the human oral cavity and the relationships between viruses and diseases such as periodontitis are scarcely deciphered. Redondoviruses were reported in the human oral cavity in 2019, including in periodontitis patients. Here, we aimed at detecting redondoviruses and at searching for a potential viral host in human saliva. Non-stimulated saliva was collected between December 2020 and June 2021. These samples were tested using real-time PCR regarding the presence of redondovirus and Entamoeba gingivalis DNA. Similarity searches were performed using BLAST against eukaryotic and prokaryotic sequences from GenBank. The redondovirus DNA was detected in 46% of the 28 human saliva samples. In addition, short fragments of redondovirus genomes were detected in silico within Entamoeba sequences. Finally, Entamoeba gingivalis DNA was detected in 46% of the 28 saliva samples, with a strong correlation between redondovirus DNA and E. gingivalis DNA detections, in 93% of the cases. Regarded together, these findings and previous ones strongly support the presence of redondoviruses in the human oral cavity and their association to E. gingivalis as their likely host. |
| Databáze: | OpenAIRE |
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