Comparison of viral glycosylation using lectin blotting with Vero cell-derived and mouse brain-derived Japanese encephalitis vaccines
| Autor: | Hiroko Toriniwa, Tomoyoshi Komiya |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Glycosylation
Biology Gene mutation Culture Media Serum-Free Mice Viral Proteins chemistry.chemical_compound Lectins Chlorocebus aethiops medicine Animals Japanese encephalitis vaccine Vero Cells Glycoproteins General Veterinary General Immunology and Microbiology Japanese Encephalitis Vaccines Immunogenicity Public Health Environmental and Occupational Health Brain Lectin Japanese encephalitis medicine.disease Virology Blot Infectious Diseases Vaccines Inactivated chemistry biology.protein Vero cell Molecular Medicine medicine.drug |
| Zdroj: | Vaccine. 29:1859-1862 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/j.vaccine.2010.12.038 |
| Popis: | We produced a Vero cell-derived inactivated Japanese encephalitis vaccine using a serum-free medium, as a substitute for the conventional mouse brain-derived Japanese encephalitis vaccine. The immunogenicity of this cell-derived vaccine was higher than that of the conventional mouse brain-derived vaccine. The results of a clinical study in humans also demonstrated higher immunogenicity of this cell-derived vaccine. No gene mutation was found in the viral structural proteins derived from Vero cells and mouse brain. So, we conducted a lectin blot analysis, assuming differing glycosylation as a cause of the higher immunogenicity in humans. The results demonstrated that vaccine reactivity varied with lectins, particularly with WGA, DBA, MAM, SSA, SBA, and GS-II. Thus, glycosylation differed with the vaccines, suggesting a possible cause of the differing immunogenicity between mice and humans. |
| Databáze: | OpenAIRE |
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