Phylogenetic, virulence and antibiotic resistance characteristics of commensal strain populations of Escherichia coli from community subjects in the Paris area in 2010 and evolution over 30 years

Autor: Catherine Branger, Françoise Jauréguy, Camille Couffignal, Azucena Mora, Alain Eddi, Anne-Sophie Daubié, Ghizlane Dahbi, Méril Massot, Bertrand Picard, Erick Denamur, Jorge Blanco, Olivier Clermont
Přispěvatelé: Infection, Anti-microbiens, Modélisation, Evolution (IAME (UMR_S_1137 / U1137)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris 13 (UP13)-Université Paris Diderot - Paris 7 (UPD7)-Université Sorbonne Paris Cité (USPC), Hôpital Avicenne [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratorio de Referencia de E. coli [Lugo, Spain] (LREC), Universidade de Santiago de Compostela [Spain] (USC ), Département de Médecine Générale - DMG [Paris] (Faculté de Médecine), Sorbonne Paris Cité-Université Paris Diderot - Paris 7 (UPD7), This work was partially supported by the grant CN2012/303(Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia and The European Regional Development Fund, ERDF)., The COLIVILLE Group. The COLIVILLE Group is composed of the following medical practitioners who were involved in the recruitment of subjects for the study: Monique Allouche, Jean-Pierre Aubert, Isabelle Aubin, Ghislaine Audran, Dan Baruch, Philippe Birembaux, Max Budowski, Emilie Chemla, Alain Eddi, Marc Frarier, Eric Galam, Julien Gelly, Serge Joly, Jean-François Millet, Michel Nougairede, Nadja Pillon, Guy Septavaux, Catherine Szwebel, Philippe Vellard, Raymond Wakim, Xavier Watelet and Philippe Zerr., Comets, Emmanuelle
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Microbiology
Microbiology, Microbiology Society, 2016, 162 (4), pp.642-50. ⟨10.1099/mic.0.000242.⟩
ISSN: 1350-0872
1465-2080
DOI: 10.1099/mic.0.000242.⟩
Popis: International audience; It is important to study commensal populations of Escherichia coli because they appear to be the reservoir of both extra-intestinal pathogenic E. coli and antibiotic resistant strains of E. coli. We studied 279 dominant faecal strains of E. coli from 243 adults living in the community in the Paris area in 2010. The phylogenetic group and sub-group [sequence type complex (STc)] of the isolates and the presence of 20 virulence genes were determined by PCR assays. The O-types and the resistance to 18 antibiotics were assessed phenotypically. The B2 group was the most frequently recovered (34.0%), followed by the A group (28.7%), and other groups were rarer. The most prevalent B2 subgroups were II (STc73), IV (STc141), IX (STc95) and I (STc131) with 22.1%, 21.1%, 16.8% and 13.7%, respectively, of the B2 group strains. Virulence factors (VFs) were more common in B2 group than other strains. One or more resistance was found in 125 strains (44.8% of the collection) but only six (2.2% of the collection) were multiresistant; no extended-spectrum beta-lactamase-producing strain was isolated. The C phylogroup and clonal group A strains were the most resistant. No trade-off between virulence and resistance was evidenced. We compared these strains to collections of strains gathered in the same conditions 30 and 10 years ago. There has been a parallel and linked increase in the frequency of B2 group strains (from 9.4% in 1980 to 22.7% in 2000 and 34.0% in 2010) and of VFs. Antibiotic resistance also increased, from 22.6% of strains resistant to at least one antibiotic in 1980 to 31.8% in 2000 and 44.8% in 2010; resistance to streptomycin, however, remained stable. Commensal human E. coli populations have clearly evolved substantially over time, presumably reflecting changes in human practices, and particularly increasing antibiotic use.
Databáze: OpenAIRE
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