The activity and expression of chitinase in the equine lung and its activity in normal horses and animals with recurrent airway obstruction
| Autor: | Laura Thompson, David Marlin, Jane A. Irwin, Christopher M. Deaton, Samantha M. Donnelly, Stephen D. Carrington, Colm J. Reid |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Biology
Gene Expression Regulation Enzymologic Pulmonary Disease Chronic Obstructive Downregulation and upregulation medicine Animals Horses Lung Recurrent airway obstruction chemistry.chemical_classification Innate immune system General Veterinary medicine.diagnostic_test Chitinases Horse respiratory system Hydrogen-Ion Concentration medicine.disease respiratory tract diseases Bronchoalveolar lavage medicine.anatomical_structure Enzyme Hexosaminidases chemistry Immunology Chitinase biology.protein RNA Horse Diseases Bronchoalveolar Lavage Fluid |
| Zdroj: | Research in veterinary science. 87(1) |
| ISSN: | 1532-2661 |
| Popis: | Recurrent airway obstruction (RAO) is a chronic inflammatory condition in equine lung, which may share a common immunological basis with human asthma, in which dysregulated Th2 responses occur. Mammals express chitinases and chitinase-like proteins, two of which are active enzymes, chitotriosidase and acidic mammalian chitinase (AMCase). Both enzymes are upregulated in a range of inflammatory conditions, including asthma. We investigated the activity of chitinase in bronchoalveolar lavage fluid from horses with and without RAO in response to organic dust challenges. No significant differences were found in activity, although in one study RAO animals had elevated chitinase activity that fell short of statistical significance. The pH optimum and pH lability of the activity was consistent with the presence of chitotriosidase. RT-PCR amplification of the mRNA encoding chitotriosidase and AMCase in normal equine lung showed that chitotriosidase, but not AMCase, is expressed in trachea, bronchi, and peripheral lung tissue. The gene for chitotriosidase was identified from the Equus caballus (horse) genome 1.1 database and its similarity to the same genes from other species was determined. The results of this study indicate that the involvement of chitotriosidase in RAO is uncertain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect