Phytochemical and Antioxidant-Related Investigations on Bark of Abies spectabilis (D. Don) Spach. from Nepal
| Autor: | Bharat Babu Shresta, Mohan B. Gewali, Pramod Kumar Jha, Emanuela Greco, Gabbriella Innocenti, Stefano Comai, Paola Minesso, Rinaldo Cervellati, Stefano Dall'Acqua |
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| Přispěvatelé: | Dall'Acqua, S, Minesso, P, Shresta, Bb, Comai, Stefano, Jha, Pk, Gewali, Mb, Greco, E, Cervellati, R, Innocenti, G., S. Dall'Acqua, P. Minesso, B.B. Shresta, S. Comai, P.K. Jha, M.B. Gewali, E. Greco, R. Cervellati, G. Innocenti |
| Rok vydání: | 2012 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Magnetic Resonance Spectroscopy DPPH Trolox equivalent antioxidant capacity Pharmaceutical Science antioxidant activity phenols Article Analytical Chemistry lcsh:QD241-441 ANTIOXIDANT CAPACITY chemistry.chemical_compound lcsh:Organic chemistry Nepal Drug Discovery phenol Organic chemistry Gallocatechin Phenols Gallic acid Physical and Theoretical Chemistry Chromatography High Pressure Liquid Plant Extracts HPLC-MS/MS Organic Chemistry Abies spectabilis NMR Abies spectabili chemistry Chemistry (miscellaneous) Polyphenol visual_art visual_art.visual_art_medium Plant Bark Molecular Medicine Bark Trolox Abies Nuclear chemistry |
| Zdroj: | Molecules Molecules; Volume 17; Issue 2; Pages: 1686-1697 Molecules, Vol 17, Iss 2, Pp 1686-1697 (2012) |
| Popis: | The bark of several coniferous species, a waste product of the timber industry, contains significant amounts of natural antioxidants. In our ongoing studies of Nepalese medicinal plants, we examined the bark from Abies spectabilis as the starting material for extracting antioxidant compounds. In vitro antioxidant activity evaluated by means of three antioxidant methods, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH), Briggs-Rauscher oscillating reaction (BR) and Trolox Equivalent Antioxidant Capacity (TEAC) and total phenol contents with the Folin-Ciocalteau reagent; the ferrous iron chelating capacity was also assessed. The methanol extract of A. spectabilis showed significant antioxidant activity and polyphenol contents (IC 50 4.13 µg/mL, 0.20 μg/mL eq. resorcinol, 4.22 mM eq. Trolox, 3.9 µg/g eq. gallic Acid in the DPPH, BR, TEAC and Folin-Ciocalteau tests, respectively) and weak Fe 2+ chelating capacity. Phytochemical studies were also carried out with 1D- and 2D NMR experiments and DI-ESI-MS, HPLC-DAD and LC-MSn measurements. Oligomeric C-type proanthocyanidins, mainly trimeric gallocatechin derivatives, were the most abundant compounds (16% of extract expressed as procyanindin B1). Gallocatechin oligomers (up to six units) and prodelphynidin-gallocatechin polymers were also identified in the extract. Prodelphynidin B4, cyclograndisolide and trans-docosanil ferulate were also isolated and characterized by NMR and MS spectroscopy. |
| Databáze: | OpenAIRE |
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