Esculetin induces cell cycle arrest and apoptosis in human colon cancer LoVo cells
| Autor: | Yong J. Choi, Chang M. Lee, Myeong Jin Nam, See-Hyoung Park |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cell cycle checkpoint
Health Toxicology and Mutagenesis Apoptosis 010501 environmental sciences Management Monitoring Policy and Law Toxicology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cyclin D1 Western blot Annexin Cell Line Tumor medicine Humans Umbelliferones Propidium iodide Cell Proliferation 0105 earth and related environmental sciences medicine.diagnostic_test Caspase 3 Chemistry Cell growth Cell Cycle Checkpoints General Medicine Cell cycle Molecular biology Proto-Oncogene Proteins c-bcl-2 030220 oncology & carcinogenesis Colonic Neoplasms embryonic structures Poly(ADP-ribose) Polymerases biological phenomena cell phenomena and immunity |
| Zdroj: | Environmental Toxicology. 34:1129-1136 |
| ISSN: | 1522-7278 1520-4081 |
| Popis: | We investigated the anti-cancer effects of ESC in human colon cancer LoVo cells. Cell counting assay results showed that ESC inhibited the proliferation of LoVo cells. Cell cycle arrest results showed that cell cycle was arrested during the G0/G1 phase in the ESC-treated LoVo cells. Western blot results showed that the cell cycle inhibitory proteins p53, p27, and p21 were increased, and cyclin D1, the cell cycle progressive protein, was decreased. Sp1 is a transcription factor regulating cell proliferation, was decreased in the ESC-treated LoVo cells. Annexin V/propidium iodide staining results showed that ESC induces apoptosis in LoVo cells. Western blot results showed that Bax, cleaved caspase -3, -7, -9, and poly(ADP-ribose) polymerase, which are proapoptotic proteins, were increased and the antiapoptotic protein Bcl-2 was decreased. Taken together, ESC induced apoptosis and has an anti-cancer effect in LoVo cells. |
| Databáze: | OpenAIRE |
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