Effects of human salivas on recombinant HIV-1 proteins
| Autor: | George K. Lewis, David W. Archibald, Kevin L. Gregory, Carla Hebert |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
0301 basic medicine
Saliva medicine.drug_class medicine.medical_treatment HIV Core Protein p24 Monoclonal antibody Salivary Glands Minor law.invention HIV Envelope Protein gp160 03 medical and health sciences fluids and secretions 0302 clinical medicine stomatognathic system law medicine Humans Parotid Gland Protease Inhibitors Protein Precursors General Dentistry Infectivity Protease Salivary gland biology Gene Products env 030206 dentistry Molecular biology Recombinant Proteins Parotid gland stomatognathic diseases 030104 developmental biology medicine.anatomical_structure Otorhinolaryngology Recombinant DNA biology.protein HIV-1 Antibody |
| Zdroj: | Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists. 4(3-4) |
| ISSN: | 1045-4411 |
| Popis: | Human saliva appears to contain factors that are inhibitory to HIV-1 infectivity in vitro. We investigated the effect of incubating human whole, parotid, labial minor salivary gland and sublingual/submandibular salivas with recombinant HIV-1 envelope protein (gpl60). Saliva/gpl60 mixtures were run on polyacrylamide gels, transferred to nitrocellulose, and assayed for the presence of gp 160 using monoclonal antibodies or HIV-1-positive sera. Incubation of the gp 160 with whole saliva reduced the intensity of gp 160 bands to 35% of control values. Minor salivary gland saliva reduced the band intensities to 65% of control values, while other saliva types diminished gp160 to 75% of control values. Protease inhibitors had no effect. Components of untreated whole human saliva prevent the detection of the HIV-1 envelope protein gp 160 by antibodies to gp120 and gp41 in immunoblots. The results suggest that complexes between whole saliva factors and certain domains of gp160 block monoclonal antibody binding or are unable to migrate through polyacrylamide gels. |
| Databáze: | OpenAIRE |
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