Tamoxifen loaded folic acid armed PEGylated magnetic nanoparticles for targeted imaging and therapy of cancer
Autor: | Alaleh Abadpour, Jaleh Barar, Mohammad-Reza Rashidi, Javid Shahbazi Mojarrad, Mostafa Heidari Majd, Yadollah Omidi, George Coukos, Davoud Asgari, Hadi Valizadeh, Efat Moumivand, Vala Kafil |
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Rok vydání: | 2013 |
Předmět: |
Stereochemistry
Breast Neoplasms Polyethylene glycol Polyethylene Glycols chemistry.chemical_compound Folic Acid Colloid and Surface Chemistry Microscopy Electron Transmission Cell Line Tumor PEG ratio Humans Physical and Theoretical Chemistry Cytotoxicity technology industry and agriculture Surfaces and Interfaces General Medicine Flow Cytometry Antineoplastic Agents Phytogenic Tamoxifen Microscopy Fluorescence chemistry Folate receptor Cancer cell Drug delivery Microscopy Electron Scanning Nanomedicine Female Drug Screening Assays Antitumor Growth inhibition Biotechnology Nuclear chemistry |
Zdroj: | Colloids and Surfaces B: Biointerfaces. 106:117-125 |
ISSN: | 0927-7765 |
DOI: | 10.1016/j.colsurfb.2013.01.051 |
Popis: | Magnetic nanoparticles (MNPs) have been widely used as drug delivery nanosystems and contrast agent for imaging and detection. To engineer multifunctional nanomedicines for simultaneous imaging and therapy of cancer cells, in the current study, we synthesized tamoxifen (TMX) loaded folic acid (FA) armed MNPs to target the folate receptor (FR) positive cancer cells. To this end, Fe3O4 nanoparticles (NPs) were synthesized through thermal decomposition of Fe(acac)3. Polyethylene glycol (PEG) was treated with excess bromoacetyl chloride (BrAc) and then with 3-aminopropyltriethoxysilane (APS) to synthesize bromoacetyl-terminal polyethylene glycol silane (APS-PEG-BrAc). The latter complex was treated with protected ethylene diamine to form a bifunctional PEG compound containing triethoxysilane at one end and amino group at the other end (APS-PEG-NH2). The Fe3O4-APS-PEG-NH2 NPs were prepared through self-assembly of APS-PEG-NH2 on MNPs, while the amino groups at the end of Fe3O4-APS-PEG-NH2 were conjugated with folic acid (FA), then loaded with TMX (Fe3O4-APS-PEG-FA-TMX). The average size of "Fe3O4-APS-PEG-FA-TMX" NPs was approximately 40 nm. The engineered MNPs were further characterized and examined in the human breast cancer MCF-7 cells that express FR. The TMX loaded MNPs (with loading efficiency of 49.1%) showed sustained liberation of TMX molecules (with 90% release in 72 h). Fluorescence microcopy and flow cytometry analyses revealed substantial interaction of Fe3O4-APS-PEG-FA-TMX NPs with the FR-positive MCF-7 cells. Cytotoxicity analysis resulted in significant growth inhibition in MCF-7 cells treated with Fe3O4-APS-PEG-FA-TMX NPs. Based on these findings, the TMX-loaded FA-armed PEGylated MNPs as a novel multifunctional nanomedicine/theranostic for concurrent targeting, imaging and therapy of the FR-positive cancer cells. |
Databáze: | OpenAIRE |
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