Primary Cultures of Human Urothelial Cells for Genotoxicity Testing
| Autor: | Klaus Golka, Angelika Flieger, Harald Schulze, Wolfram Föllmann |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Time Factors Adolescent Cell Survival Health Toxicology and Mutagenesis Urinary system Urinary Bladder Cell Culture Techniques Intermediate Filaments Biology Toxicology Cytokeratin Ureter medicine Humans Urothelium Child Cells Cultured Aged Cell Proliferation Aged 80 and over Confluency Urinary bladder L-Lactate Dehydrogenase Mutagenicity Tests Epithelial Cells Middle Aged medicine.anatomical_structure Gene Expression Regulation Cell culture Immunohistochemistry Female |
| Zdroj: | Journal of Toxicology and Environmental Health, Part A. 71:930-935 |
| ISSN: | 1087-2620 1528-7394 |
| Popis: | A cell culture system with human-derived urothelial cells was established based upon previous experience with cultures of porcine urinary bladder epithelial cells. Human tissue specimens used were derived from urinary bladders (n = 17) or ureters (n = 50) of patients undergoing urological operations. The epithelial origin and differentiation status was evaluated by an immunohistochemical staining for cytokeratins 7, 8, 18, 19, and 20 for isolated and cultured cells. Specimens from human ureters were better suited for primary cell cultures of the urothelium than specimens from human urinary bladders. Successful attachment and proliferation were reached by 98% of the ureter specimens (urinary bladder: 71%) and confluency was reached by 78% of the ureter cultures (urinary bladder: 18%). In the first 14 d of culture the cytokeratin patterns of cultured cells were comparable to those of native mucosa cells. During prolonged cell culture the cytokeratin patterns of the human urothelial cells (HUC) changed into a beginning dedifferentiation: Cytokeratin (CK) 18 was only detectable in cell cultures cultured for more than 29 d, whereas CK 19 was not detectable at d 29. Cell cultures of primary human urothelial cells may be used for in vitro testing of cytotoxic or genotoxic effects. |
| Databáze: | OpenAIRE |
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