Ontogenetic changes in energetic reserves, digestive enzymes, amino acid and energy content of Lithodes santolla (Anomura: Lithodidae): Baseline for culture
| Autor: | Gustavo A. Lovrich, Jesica Romina Mufari, Claudia Clementina Boy, Rodrigo A. Lorenzo, Hernán Javier Sacristán |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Glycogens Muscle Physiology Arginine Physiology Lithodes santolla Glycobiology Muscle Proteins Crabs Aquaculture Biochemistry chemistry.chemical_compound Aromatic Amino Acids Hemolymph Medicine and Health Sciences Amino Acids chemistry.chemical_classification 0303 health sciences Multidisciplinary Glycogen Organic Compounds Muscles Tryptophan Gene Expression Regulation Developmental Eukaryota Muscle Biochemistry 04 agricultural and veterinary sciences Lipids Amino acid Crustaceans Enzymes Chemistry Physical Sciences Medicine Female Digestive Enzymes Research Article Arthropoda Science Zoology Biology Gene Expression Regulation Enzymologic Arthropod Proteins 03 medical and health sciences Animals 030304 developmental biology Methionine Organic Chemistry Organisms Chemical Compounds Biology and Life Sciences Proteins Midgut biology.organism_classification Animal Feed Invertebrates Diet Optogenetics chemistry 040102 fisheries Enzymology 0401 agriculture forestry and fisheries Anomura Energy Metabolism |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 5, p e0232880 (2020) |
| ISSN: | 1932-6203 |
| Popis: | The southern king crab (SKC) Lithodes santolla is an important commercial species in southern South America. Fishing pressure has caused the deterioration of its stocks. Currently, culture techniques are being developed for producing SKC juveniles to enhance the natural population and to recover the fishing stock. Therefore, it is necessary to know about physiology, energetic and nutritional requirements for SKC maintenance in hatchery. Thus, this study aims to evaluate the biochemical and physiological changes in the midgut gland, muscle and hemolymph of juveniles, pre-adults and adults of wild SKC. The energetic reserves, digestive enzymes activity, amino acid profile and energy were quantified in twelve juveniles, ten pre-adult, and ten adult crabs. Juveniles showed high glycogen and low lipids in the midgut gland, and low proteins and low lactate in muscle. In the hemolymph, juveniles had high lipids. Pre-adults had high glycogen and lipids in the midgut gland, and both high protein and lactate in muscle. In the hemolymph, pre-adults had high lipids. Adults had low glycogen and high lipids in midgut gland, and both high proteins and high lactate in muscle. In hemolymph, adults had high glucose and lactate. Juveniles and pre-adults had high proteinase activity, whereas adults had high lipase activity. Major essential amino acids of SKC were arginine, methionine, and tryptophan, and the non-essential amino acids were glycine, aspartic acid and glutamic acid. On another hand, SKC had similar energy in the midgut gland and muscle, regardless of the ontogenetic stage. Moreover, we demonstrated that the biochemical energy calculation underestimates the actual measured values by a calorimeter. Thus, our results help to understand the physiological changes, energetic and nutritional requirements of L. santolla, and this study is a baseline for research on diet formulation for maintaining this species under culture conditions. |
| Databáze: | OpenAIRE |
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