A consensus Alu repeat probe for physical mapping
| Autor: | Mark A. Batzer, Prescott L. Deininger, Michelle Alegria-Hartman |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Molecular Sequence Data
Alu element Computational biology Hybrid Cells Biology Polymerase Chain Reaction Applied Microbiology and Biotechnology Genome DNA sequencing law.invention Nucleic acid thermodynamics law Consensus Sequence Genetics Consensus sequence Animals Humans Polymerase chain reaction DNA Primers Repetitive Sequences Nucleic Acid Base Sequence Genome Human Chromosome Mapping Nucleic Acid Hybridization Genetic Techniques Indicators and Reagents Human genome Physical mapping DNA Probes |
| Zdroj: | Genetic Analysis: Biomolecular Engineering. 11:34-38 |
| ISSN: | 1050-3862 |
| Popis: | Physical mapping of the human genome involves a variety of complex hybridization-based procedures, some of which rely upon the ability to separate human clones derived from human-rodent hybrid cell lines from those that contain background rodent-derived DNA sequences. The ability to block the repetitive element (Alu repeat) portion of inter-Alu PCR products derived from a variety of complex sources is also crucial for the isolation of unique DNA sequences. Here we report the construction and characterization of a new consensus Alu repeat probe (pPD39) designed for these purposes. |
| Databáze: | OpenAIRE |
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