Quantification by SIFT-MS of volatile compounds produced by the action of sodium hypochlorite on a model system of infected root canal content
| Autor: | Sadia Niazi, Claire Turner, Francesco Mannocci, Konstantinos Ioannidis, David W. Smith, Sanjukta Deb |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Teeth
Sodium Hypochlorite Hazardous Substances/analysis Sodium Hypochlorite/chemistry Root canal Dental and Oral Procedures lcsh:Medicine Transportation Biochemistry 01 natural sciences Mass Spectrometry chemistry.chemical_compound 0302 clinical medicine Bacteria/chemistry Medicine and Health Sciences lcsh:Science Disinfection/methods Multidisciplinary Chloroform Hypochlorites biology Organic Compounds Transportation Infrastructure Chemistry medicine.anatomical_structure Sodium hypochlorite Physical Sciences Ultrapure water Engineering and Technology Root Canal Irrigants/chemistry Anatomy Chlorine Root Canal Preparation Research Article Chemical Elements Root Canal Preparation/methods chemistry.chemical_element Surgical and Invasive Medical Procedures Actinomyces radicidentis Civil Engineering Hazardous Substances Enterococcus faecalis 03 medical and health sciences Albumins medicine Humans Volatile Organic Compounds/analysis Mass Spectrometry/methods Serum Albumin Volatile Organic Compounds Chromatography Bacteria Root Canal Irrigants lcsh:R Organic Chemistry 010401 analytical chemistry Chemical Compounds Biology and Life Sciences Proteins 030206 dentistry biology.organism_classification 0104 chemical sciences Disinfection Jaw chemistry Dentin Canals lcsh:Q Salts Selected-ion flow-tube mass spectrometry Digestive System Head |
| Zdroj: | PLoS ONE Ioannidis, K, Niazi, S, Deb, S, Mannocci, F, Smith, D & Turner, C 2018, ' Quantification by SIFT-MS of volatile compounds produced by the action of sodium hypochlorite on a model system of infected root canal content ', PLoS ONE, vol. 13, no. 9, e0198649, pp. e0198649 . https://doi.org/10.1371/journal.pone.0198649 PLoS ONE, Vol 13, Iss 9, p e0198649 (2018) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0198649 |
| Popis: | Root canal irrigation with sodium hypochlorite (NaOCl) is an indispensable part of the chemomechanical preparation of infected root canals in Endodontology. However, there is limited information on the emergence of toxic or hazardous volatile compounds (VOCs) from the interaction of NaOCl with the infected content of tooth biomaterials. The aim of this study was to assess the formation of VOCs and disinfection by-products (DBPs) following the interaction of NaOCl 2.5% v/v with a model system of different sources of natural organic matter (NOM) present in infected root canals, including dentine powder, planktonic multi-microbial suspensions (Propionibacterium acnes, Staphylococcus epidermidis, Actinomyces radicidentis, Streptococcus mitis and Enterococcus faecalis strain OMGS3202), bovine serum albumin 4%w/v and their combination. NaOCl was obtained from a stock solution with iodometric titration. Ultrapure water served as negative control. Samples were stirred at 37°C in aerobic and anaerobic conditions for 30min to approximate a clinically realistic time. Centrifugation was performed and the supernatants were collected and stored at -800 C until analysis. The reaction products were analysed in real time by selected ion flow tube mass spectrometry (SIFT-MS) in triplicates. SIFT-MS analysis showed that the released VOCs included chlorinated hydrocarbons, particularly chloroform, together with unexpected higher levels of some nitrogenous compounds, especially acetonitrile. No difference was observed between aerobic and anaerobic conditions. The chemical interaction of NaOCl with NOM resulted in the formation of toxic chlorinated VOCs and DBPs. SIFT-MS analysis proved to be an effective analytical method. The risks from the rise of toxic compounds require further consideration in dentistry. |
| Databáze: | OpenAIRE |
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