Boosting anti-idiotype immune response with recombinant AAV enhances tumour protection induced by gene gun vaccination

Autor: L Zentilin, Mauro Giacca, M. Cesco-Gaspere, Oscar R. Burrone
Přispěvatelé: M., Cesco Gaspere, L., Zentilin, Giacca, Mauro, O. R., Burrone
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Idiotype
Lymphoma
viruses
Gene gun
immunology/therapy
Mice
Transduction
immunology
administration /&/ dosage/genetics/immunology
Transduction (genetics)
Mice
immunology
Antigen
Transduction
Genetic
Vaccines
DNA
genetics
immunology
Dependoviru
methods
Cancer Vaccine
Intramuscular
Vaccines
biology
Genetic
Vaccine
Subcutaneous
General Medicine
Dependovirus
Subcutaneous
Lymphoma
Antibodies
Anti-Idiotypic
immunology/therapy
Animals
Antibodies
Anti-Idiotypic
immunology
Antigens
Neoplasm
immunology
Biolistics
methods
Cancer Vaccines
immunology
Dependovirus
genetics
Genetic Vectors
Humans
Immunoglobulin Idiotypes
administration /&/ dosage/genetics/immunology
Injections
Intramuscular
Injections
Intraperitoneal
Injections
B-Cell
Genetic
Vaccines
DNA
Naked DNA
Animals
Antibodie
Antibody
Injections
Intraperitoneal
Intramuscular
Injection
Lymphoma
B-Cell
immunology
Biolistic
Injections
Subcutaneous
Genetic Vectors
genetics
Genetic Vectors
Humans
Immunoglobulin Idiotype
administration /&/ dosage/genetics/immunology
Injection
Injections
Intramuscular
Cancer Vaccines
Antibodies
methods
Injections
Transduction
Immune system
Antigen
Immunoglobulin Idiotypes
Genetic
Antigens
Neoplasm
In vivo
Animals
Humans
Intraperitoneal
Antigens
Biolistics
Virology
Immunology
biology.protein
Intraperitoneal
Injection
Popis: Thanks to the safety of administration, efficiency of in vivo transduction and persistence of transgene expression, vectors based on the adeno-associated virus (AAV) are extensively utilized in both preclinical and clinical experimentation. Here we thoroughly explore the potential of AAV-mediated antigen delivery for tumour vaccination. A recombinant AAV vector (rAAV) encoding a lymphoma idiotype (Id) in a single-chain variable fragment format was found to induce an efficient anti-Id immune response upon injection in immunocompetent animals. The intensity of the immune response and the protective effect of rAAV administration in vivo were systematically compared with those elicited by simple injection of naked DNA or biolistic immunization. The results indicate that Id delivery via rAAV enhances the intensity of immune response compared with injection of naked DNA, while anti-idiotypic antibodies titres are not considerably increased compared with biolistic vaccination. On the contrary, a prime-boost vaccination strategy combining biolistic and AAV DNA delivery results in a major increase in anti-Id antibody response compared with the repetitive biolistic immunization. This increased anti-Id humoral response strictly correlated with a significant improvement on tumour protection in vivo.
Databáze: OpenAIRE
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