Detection of intracellular interleukin-10 by flow cytometry
| Autor: | V. Rubin Kelley, Gary G. Singer, Cristina Diaz-Gallo, Burkhard Kreft |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Cytoplasm
Cell Membrane Permeability medicine.medical_treatment T cell Immunology Biology In Vitro Techniques Flow cytometry Cell membrane chemistry.chemical_compound Mice medicine Immunology and Allergy Animals medicine.diagnostic_test Interleukin Flow Cytometry Molecular biology Cell biology Interleukin-10 Interleukin 10 Digitonin medicine.anatomical_structure Cytokine chemistry Intracellular |
| Zdroj: | Journal of immunological methods. 156(1) |
| ISSN: | 0022-1759 |
| Popis: | Detection of cytokines is limited to measurements of the secreted molecule. To circumvent this problem we permeabilized the cell membrane with digitonin and localize cytokine expression using antibodies by flow cytometry. In this report we demonstrate that we can detect specific intracellular interleukin-10 (IL-10) in the HT-2 T cell line only after membrane permeabilization. With this technique intracellular cytokines are readily detectable. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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