Partial purification and characterization of serine protease produced through fermentation of organic municipal solid wastes by Serratia marcescens A3 and Pseudomonas putida A2
| Autor: | Kamrul Islam, Md. Assaduzzaman, Abul Kalam Azad, Asif Iqbal, Mohammad Rejaur Rahman, Md. Faisal Azim, Al Hakim, Jahed Ahmed, Md. Mozammel Hoq, Md. Saddam Hossain |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine lcsh:QH426-470 medicine.medical_treatment lcsh:Biotechnology 01 natural sciences Serine 03 medical and health sciences 010608 biotechnology lcsh:TP248.13-248.65 medicine General Materials Science Serine protease Protease Chromatography biology Molecular mass biology.organism_classification Pseudomonas putida lcsh:Genetics 030104 developmental biology Biochemistry Serratia marcescens biology.protein Fermentation Bacteria |
| Zdroj: | Journal of Genetic Engineering and Biotechnology, Vol 16, Iss 1, Pp 29-37 (2018) |
| Popis: | Proteolytic bacteria isolated from municipal solid wastes (MSW) were identified as Serratia marcescens A3 and Pseudomonas putida A2 based on 16S rDNA sequencing. Protease produced through fermentation of organic MSW by these bacteria under some optimized physicochemical parameters was partially purified and characterized. The estimated molecular mass of the partially purified protease from S. marcescens and P. putida was approximately 25 and 38 kDa, respectively. Protease from both sources showed low Km 0.3 and 0.5 mg ml−1 and high Vmax 333 and 500 µmole min−1 at 40 °C, and thermodynamics analysis suggested formation of ordered enzyme-substrate (E-S) complexes. The activation energy (Ea) and temperature quotient (Q10) of protease from S. marcescens and P. putida were 16.2 and 19.9 kJ/mol, and 1.4 and 1.3 at temperature range from 20 to 40 °C, respectively. Protease of the both bacterial isolates was serine and cysteine type. The protease retained approximately 97% of activity in the presence of sodium dodecyl sulphate. It was observed that the purified protease of S. marcescens could remove blood stains from white cotton cloth and degrade chicken flesh remarkably. Our study revealed that organic MSW can be used as raw materials for bacterial protease production and the protease produced by S. marcescens A3 might be potential for applications. Keywords: Municipal solid wastes, Purified protease, Activation energy, Kinetics, Blood stains |
| Databáze: | OpenAIRE |
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