Diversity of oat varieties in eliciting the early inflammatory events in celiac disease
| Autor: | Marco Silano, Patrizia Restani, Sara Manferdelli, Francesca Uberti, Elena Penas Pozo, Olimpia Vincentini, Andrea Budelli, Cristina Felli, Tamara Del Pinto |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Avena Duodenum Medicine (miscellaneous) Disease Autoimmune enteropathy Biology digestive system Oats Gliadin Diet Gluten-Free GTP-Binding Proteins Cell Line Tumor medicine otorhinolaryngologic diseases Humans Protein Glutamine gamma Glutamyltransferase 2 Phosphorylation Child chemistry.chemical_classification Nutrition and Dietetics Transglutaminases Avenins fungi nutritional and metabolic diseases food and beverages Original Contribution medicine.disease Gluten digestive system diseases Celiac Disease Biochemistry chemistry Immunology Gluten-free diet Gluten free Electrophoresis Polyacrylamide Gel Female K562 Cells |
| Zdroj: | European Journal of Nutrition |
| ISSN: | 1436-6215 |
| Popis: | Purpose Celiac disease (CD) is an autoimmune enteropathy, triggered by dietary gluten. The only treatment is a strict gluten-free diet. Oats are included in the list of gluten-free ingredients by European Regulation, but the safety of oats in CD is still a matter of debate. The present study examined the capability of different oat cultivars of activating the gliadin-induced transglutaminase-2 (TG2)-dependent events in some in vitro models of CD. In addition, we compared this capability with the electrophoresis pattern of peptic–tryptic digests of the proteins of the oat cultivars. Methods K562(S) cells agglutination, transepithelial electrical resistance of T84-cell monolayers, intracellular levels of TG2 and phosphorylated form of protein 42–44 in T84 cells were the early gliadin-dependent events studied. Results The results showed that the Nave oat cultivar elicited these events, whereas Irina and Potenza varieties did not. The ability of a cultivar to activate the above-described events was associated with the electrophoretic pattern of oat proteins and their reactivity to anti-gliadin antibodies. Conclusion We found significant differences among oat cultivars in eliciting the TG2-mediated events of CD inflammation. Therefore, the safety of an oat cultivar in CD might be screened in vitro by means of biochemical and biological assays, before starting a clinical trial to definitely assess its safety. |
| Databáze: | OpenAIRE |
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