Teething during sleep: Ultrastructural analysis of pharyngeal muscle and cuticular grinder during the molt in Caenorhabditis elegans
| Autor: | David M. Raizen, Matthew D. Nelson, Nicholas F. Trojanowski, Alessandro P. Sparacio, Karen M. Snetselaar |
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| Rok vydání: | 2020 |
| Předmět: |
Teeth
Muscle Physiology Nematoda Physiology Respiratory System Cell Membranes Molting Tooth Eruption Extracellular matrix 0302 clinical medicine Medicine and Health Sciences Musculoskeletal System Caenorhabditis elegans 0303 health sciences Multidisciplinary biology Chemistry Muscles Metalloendopeptidases Eukaryota Animal Models Cell biology medicine.anatomical_structure Experimental Organism Systems Larva Pharyngeal Muscles Medicine Anatomy Cellular Structures and Organelles Moulting Research Article Muscle Contraction Science Research and Analysis Methods Time-Lapse Imaging Pharyngeal muscles 03 medical and health sciences Model Organisms Microscopy Electron Transmission Extracellular medicine Animals Adults Vesicles Sarcomere organization Caenorhabditis elegans Proteins 030304 developmental biology Pharynx Organisms Biology and Life Sciences Cell Biology biology.organism_classification Invertebrates Jaw Age Groups People and Places Animal Studies Caenorhabditis Ultrastructure Population Groupings Sleep Digestive System Head 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE, Vol 15, Iss 5, p e0233059 (2020) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Complex extracellular structures exist throughout phylogeny, but the dynamics of their formation and dissolution are often opaque. One example is the pharyngeal grinder of the nematode Caenorhabditis elegans, an extracellular structure that ruptures bacteria during feeding. During each larval transition stage, called lethargus, the grinder is replaced with one of a larger size. Here, we characterize at the ultrastructural level the deconstruction of the larval grinder and the construction of the adult grinder during the fourth larval stage (L4)-to-adult transition. Early in L4 lethargus, pharyngeal muscle cells trans-differentiate from contractile to secretory cells, as evidenced by the appearance of clear and dense core vesicles and disruptions in sarcomere organization. This is followed, within minutes, by the dissolution of the L4 grinder and the formation and maturation of the adult grinder. Components of the nascent adult grinder are deposited basally, and are separated from the dissolving larval grinder by a visible apical layer. The complete grinder is a lamellated extracellular matrix comprised of five layers. Following grinder formation, pharyngeal muscle cells regain ultrastructural contractile properties, and muscle contractions resume. Our findings add to our understanding of how complex extracellular structures assemble and dissemble. |
| Databáze: | OpenAIRE |
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