Acrylamide induces a thyroid allostasis–adaptive response in prepubertal exposed rats

Autor: Isabela Medeiros de Oliveira, Dalton Luiz Schiessel, Fernanda Ivanski, Viviane Matoso de Oliveira, Paula Bargi-Souza, Marco Romano, Renata Marino Romano
Jazyk: angličtina
Rok vydání: 2020
Předmět:
DIO1
iodothyronine deiodinase 1
PND
postnatal day
Health
Toxicology and Mutagenesis
Metabolite
Toxicology
Applied Microbiology and Biotechnology
chemistry.chemical_compound
NOAEL
no Observed Adverse Effect Level
TSH
thyrotropin
TH
thyroid hormones
TDI
tolerable daily intake
TSH
thyroid hormone receptor
TPO
thyroid peroxidase
HPT
hypothalamus-pituitary-thyroid axis
DIO3
iodothyronine deiodinase 3
Thyroid
Acrylamide
Chemistry
AA
acrylamide
BW
body weight
Allostasis
Hypothalamic–pituitary–thyroid axis
medicine.anatomical_structure
MCT-8
monocarboxylate transporter 8
Hypothalamus
Thyroid hormone metabolism
RfD
reference dose
medicine.medical_specialty
TRH
thyrotropin releasing hormone hormone
Article
DIO2
iodothyronine deiodinase 2
lcsh:RA1190-1270
Internal medicine
THRA1
thyroid hormone receptor alpha 1
medicine
EDCs
endocrine-disrupting chemicals
T4
thyroxine
Endocrine system
lcsh:Toxicology. Poisons
ComputingMethodologies_COMPUTERGRAPHICS
LDL
low lipoproteins
T3
triiodothyronine
Endocrine-disrupting chemicals
HDL
high-density lipoproteins
THRB2
thyroid hormone receptor beta 2
LOAEL
lowest Observed Adverse Effect Level
TRHR
thyrotropin releasing hormone receptor
Endocrinology
MYH6
myosin heavy chain 6
PDS
pendrin
Homeostasis
NIS
sodium/iodide symporter
Hormone
Zdroj: Current Research in Toxicology
Current Research in Toxicology, Vol 1, Iss, Pp 124-132 (2020)
ISSN: 2666-027X
Popis: Graphical abstract
Highlights • Acrylamide acts as endocrine disruptor for the thyroid gland function. • Acrylamide increases the transcript expression of proteins related to THs synthesis. • Exposure to acrylamide alters the hypothalamus-pituitary-thyroid axis homeostasis. • Acrylamide induces allostatic regulation of the hypothalamus-pituitary-thyroid axis.
Some endocrine-disrupting chemicals (EDCs) can affect the endocrine system through covalent interactions with specific sites, leading to deregulation of physiological homeostasis. The acrylamide (AA) present in some fried or baked foods is an example of an electrophile molecule that is able to form adducts with nucleophilic regions of nervous system proteins leading to neurological defects. A positive correlation between increased urinary AA metabolite concentration and reduced levels of thyroid hormones (TH) was described in adolescents and young adults. Thus, this study aimed to evaluate whether AA affects the physiology of the hypothalamus-pituitary-thyroid (HPT) axis and the possible repercussions in peripheral TH-target systems. For this, male Wistar rats were exposed to doses of 2.5 or 5.0 mg AA/Kg/day, based on the LOAEL (Lowest Observed Adverse Effect Level) during prepubertal development. The expression of molecular markers of HPT functionality was investigated in the hypothalamus, pituitary, thyroid, heart and liver, as well as the hormonal and lipid profiles in blood samples. Herein, we showed that AA acts as EDCs for thyroid gland function, increasing the transcript expression of several proteins related to TH synthesis and altering hypothalamus-pituitary-thyroid axis homeostasis, an effect evidenced by the higher levels of THs in the serum. Compensatory mechanisms were observed in TH-target tissues, such as an increase in Dio3 mRNA expression in the liver and a reduction in Mct8 transcript content in the hearts of AA-treated rats. Together, these results pointed out an allostatic regulation of the HPT axis induced by AA and suggest that chronic exposure to it, mainly associated with food consumption, might be related to the higher prevalence of thyroid dysfunctions.
Databáze: OpenAIRE
Externí odkaz: