A Macrocyclic Fluorophore Dimer with Flexible Linkers: Bright Excimer Emission with a Long Fluorescence Lifetime
| Autor: | Chih-Ming Chou, Kai Welke, Tetsuya Higashiyama, Shigehiro Yamaguchi, Yoshikatsu Sato, Masayasu Taki, Hiroshi Osaki, Aiko Fukazawa, Shohei Saito, Stephan Irle, Daisuke Yokogawa |
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| Rok vydání: | 2016 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Anthracene Brightness Fluorophore 010405 organic chemistry Dimer Analytical chemistry 02 engineering and technology General Medicine General Chemistry 021001 nanoscience & nanotechnology 010402 general chemistry Photochemistry Excimer 01 natural sciences Fluorescence Catalysis 0104 chemical sciences chemistry.chemical_compound chemistry Molecule 0210 nano-technology |
| Zdroj: | Angewandte Chemie International Edition. 55:7131-7135 |
| ISSN: | 1433-7851 |
| Popis: | Bright fluorescent molecules with long fluorescence lifetimes are important for the development of lifetime-based fluorescence imaging techniques. Herein, a molecular design is described for simultaneously attaining long fluorescence lifetime (τ) and high brightness (ΦF ×ɛ) in a system that features macrocyclic dimerization of fluorescent π-conjugated skeletons with flexible linkers. An alkylene-linked macrocyclic dimer of bis(thienylethynyl)anthracene was found to show excimer emission with a long fluorescence lifetime (τ≈19 ns) in solution, while maintaining high brightness. A comparison with various relevant derivatives revealed that the macrocyclic structure and the length of the alkylene chains play crucial roles in attaining these properties. In vitro time-gated imaging experiments were conducted as a proof-of-principle for the superiority of this macrocyclic fluorophore relative to the commercial fluorescent dye Alexa Fluor 488. |
| Databáze: | OpenAIRE |
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