A Macrocyclic Fluorophore Dimer with Flexible Linkers: Bright Excimer Emission with a Long Fluorescence Lifetime

Autor: Chih-Ming Chou, Kai Welke, Tetsuya Higashiyama, Shigehiro Yamaguchi, Yoshikatsu Sato, Masayasu Taki, Hiroshi Osaki, Aiko Fukazawa, Shohei Saito, Stephan Irle, Daisuke Yokogawa
Rok vydání: 2016
Předmět:
Zdroj: Angewandte Chemie International Edition. 55:7131-7135
ISSN: 1433-7851
Popis: Bright fluorescent molecules with long fluorescence lifetimes are important for the development of lifetime-based fluorescence imaging techniques. Herein, a molecular design is described for simultaneously attaining long fluorescence lifetime (τ) and high brightness (ΦF ×ɛ) in a system that features macrocyclic dimerization of fluorescent π-conjugated skeletons with flexible linkers. An alkylene-linked macrocyclic dimer of bis(thienylethynyl)anthracene was found to show excimer emission with a long fluorescence lifetime (τ≈19 ns) in solution, while maintaining high brightness. A comparison with various relevant derivatives revealed that the macrocyclic structure and the length of the alkylene chains play crucial roles in attaining these properties. In vitro time-gated imaging experiments were conducted as a proof-of-principle for the superiority of this macrocyclic fluorophore relative to the commercial fluorescent dye Alexa Fluor 488.
Databáze: OpenAIRE