Inhibition of Functional T Cell Priming and Contact Hypersensitivity Responses by Treatment with Anti-Secondary Lymphoid Chemokine Antibody During Hapten Sensitization
| Autor: | Robert L. Fairchild, Anton V. Gorbachev, Ronald P. Gladue, Tara M. Engeman, Peter S. Heeger |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Chemokine
Langerhans cell T-Lymphocytes T cell Immunology Priming (immunology) Inflammation Dermatitis Contact Lymphocyte Activation Mice Immune Tolerance medicine Animals Immunology and Allergy Sensitization Mice Inbred BALB C Chemokine CCL21 integumentary system biology Chemistry Oxazolone Antibodies Monoclonal Dendritic cell Adoptive Transfer medicine.anatomical_structure Chemokines CC Langerhans Cells Cell Migration Inhibition Injections Intravenous biology.protein Cytokines Dinitrofluorobenzene Female Immunization medicine.symptom Haptens Hapten |
| Zdroj: | The Journal of Immunology. 164:5207-5214 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.164.10.5207 |
| Popis: | Recent studies have suggested a pivotal role for secondary lymphoid chemokine (SLC) in directing dendritic cell trafficking from peripheral to lymphoid tissues. As an extension of these studies, we examined the consequences of anti-SLC Ab treatment during Ag priming on T cell function in an inflammatory response. We used a model of T cell-mediated inflammation, contact hypersensitivity (CHS), where priming of the effector T cells is dependent upon epidermal dendritic cell, Langerhans cells, and migration from the hapten sensitization site in the skin to draining lymph nodes. A single injection of anti-SLC Ab given at the time of sensitization with FITC inhibited Langerhans cell migration into draining lymph nodes for at least 3 days. The CHS response to hapten challenge was inhibited by anti-SLC Ab treatment in a dose-dependent manner. Despite the inhibition of CHS, T cells producing IFN-γ following in vitro stimulation with anti-CD3 mAb or with hapten-labeled cells were present in the skin-draining lymph nodes of mice treated with anti-SLC Ab during hapten sensitization. These T cells were unable, however, to passively transfer CHS to naive recipients. Animals treated with anti-SLC Ab during hapten sensitization were not tolerant to subsequent sensitization and challenge with the hapten. In addition, anti-SLC Ab did not inhibit CHS responses when given at the time of hapten challenge. These results indicate an important role for SLC during sensitization for CHS and suggest a strategy to circumvent functional T cell priming for inflammatory responses through administration of an Ab inhibiting dendritic cell trafficking. |
| Databáze: | OpenAIRE |
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