Derivation of human induced pluripotent stem cell (iPSC) line with LRRK2 gene R1398H variant in Parkinson's disease

Autor: Shin Hui Ng, Li Zeng, Christina Ying Yan Lim, Y. Zhao, Dongrui Ma, Murni Tio, Eng-King Tan
Rok vydání: 2017
Předmět:
0301 basic medicine
Parkinson's disease
Genotype
Cellular differentiation
Genetic enhancement
DNA Mutational Analysis
Genetic Vectors
Induced Pluripotent Stem Cells
Karyotype
Germ layer
Mice
SCID
Biology
Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
Peripheral blood mononuclear cell
Polymorphism
Single Nucleotide
Sendai virus
Cell Line
03 medical and health sciences
Mice
0302 clinical medicine
Mice
Inbred NOD
medicine
Animals
Humans
Induced pluripotent stem cell
Aged
Medicine(all)
Base Sequence
Teratoma
Cell Differentiation
Parkinson Disease
General Medicine
Cell Biology
medicine.disease
Cellular Reprogramming
Molecular biology
030104 developmental biology
Microscopy
Fluorescence
Cell culture
Cancer research
Leukocytes
Mononuclear
Female
Reprogramming
030217 neurology & neurosurgery
Transcription Factors
Developmental Biology
Zdroj: Stem Cell Research. 18:48-50
ISSN: 1873-5061
DOI: 10.1016/j.scr.2016.12.014
Popis: Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 72-year old female Parkinson's disease (PD) patient with R1398H variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model provides a good platform for studying the mechanism of PD, and also for drug testing and gene therapy studies.
Databáze: OpenAIRE
Externí odkaz: