Molecular basis of species-specific expression of repolarizing K+currents in the heart
| Autor: | Isaac L. Moss, Julius Gy. Papp, Stanley Nattel, Robert Dumaine, András Varró, Charles Antzelevich, Bruce G. Allen, Stephen Zicha |
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| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Potassium Channels Physiology Protein subunit Blotting Western Guinea Pigs Biology K currents Antiarrythmic drugs Species Specificity Physiology (medical) Internal medicine Gene expression medicine Animals Humans RNA Messenger Ion channel Shal Potassium Channels Reverse Transcriptase Polymerase Chain Reaction Myocardium Myocardium metabolism Electrophysiology Endocrinology Biophysics Rabbits Cardiology and Cardiovascular Medicine |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 285:H1641-H1649 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.00346.2003 |
| Popis: | There are important species-specific differences in K+current profiles and arrhythmia susceptibility, but interspecies comparisons of K+channel subunit expression are lacking. We quantified voltage-gated K+channel (Kv) subunit mRNA and protein in rabbits, guinea pigs, and humans. Kv1.4, Kv4.2, and Kv4.3 mRNA was present in rabbits but undetectable in guinea pigs. MinK mRNA concentration in guinea pigs was almost threefold greater versus humans and 20-fold versus rabbits. MinK protein expression in guinea pigs was almost twofold that in humans and sixfold that in rabbits. KvLQT1 mRNA concentration was greatest in humans, and protein expression in humans was increased by ∼2- and ∼7-fold compared with values in rabbits and guinea pigs, respectively. The ether-a-go-go-related gene (ERG1) mRNA was more concentrated in humans, but ERG1 protein expression could not be compared across species because of epitope sequence differences. We conclude that important interspecies differences in cardiac K+channel subunit expression exist and may contribute to the following: 1) lack of a transient outward current in the guinea pig (α-subunit transcription absent in the guinea pig heart); 2) small slow delayed rectifier current and torsades de pointes susceptibility in the rabbit (low-level minK expression); and 3) large slow component of the delayed rectifier current in the guinea pig (strong minK expression). |
| Databáze: | OpenAIRE |
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