TFAM-deficient mouse skin fibroblasts – an ex vivo model of mitochondrial dysfunction
| Autor: | Carolina Meroño, Manuel J. Del Rey, Cristina Municio, María Mittelbrunn, Inés García-Consuegra, José L. Pablos, Alicia Usategui, Gabriel Criado |
|---|---|
| Přispěvatelé: | Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España) |
| Rok vydání: | 2021 |
| Předmět: |
Mitochondrial DNA
Stromal cell Neuroscience (miscellaneous) Medicine (miscellaneous) Cellular senescence DNA Mitochondrial General Biochemistry Genetics and Molecular Biology Mitochondrial Proteins Mice Immunology and Microbiology (miscellaneous) fibroblasts mitochondrial dysfunction Pathology Animals cellular senescence RB1-214 Glycolysis TFAM Inflammation Messenger RNA Chemistry High Mobility Group Proteins Fibroblasts Mitochondria tfam Cell biology Organoids DNA-Binding Proteins Mitochondrial respiratory chain Gene Expression Regulation inflammation Respirasome Medicine Mitochondrial dysfunction Ex vivo Research Article |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Disease Models & Mechanisms, Vol 14, Iss 8 (2021) Disease Models & Mechanisms article-version (VoR) Version of Record |
| Popis: | Mitochondrial dysfunction associates with several pathological processes and contributes to chronic inflammatory and ageing-related diseases. Mitochondrial transcription factor A (TFAM) plays a critical role in maintaining mtDNA integrity and function. Taking advantage of Tfamfl/fl UBC-Cre/ERT2+/+ mice to investigate mitochondrial dysfunction in the stromal cell component, we describe an inducible in vitro model of mitochondrial dysfunction by stable depletion of TFAM in primary mouse skin fibroblasts (SK-FBs) after 4-hydroxytamoxifen (4-OHT) administration. Tfam gene deletion caused a sustained reduction in Tfam and mtDNA-encoded mRNA in Cre(+) SK-FBs cultured for low (LP) and high (HP) passages that translated into a loss of TFAM protein. TFAM depletion led to a substantial reduction in mitochondrial respiratory chain complexes that was exacerbated in HP SK-FB cultures. The assembly pattern showed that the respiratory complexes fail to reach the respirasome in 4-OHT-treated Cre(+) SK-FBs. Functionally, mito-stress and glycolysis-stress tests showed that mitochondrial dysfunction developed after long-term 4-OHT treatment in HP Cre(+) SK-FBs and was compensated by an increase in the glycolytic capacity. Finally, expression analysis revealed that 4-OHT-treated HP Cre(+) SK-FBs showed a senescent and pro-inflammatory phenotype. Summary: TFAM depletion in skin fibroblasts ex vivo results in a senescent and inflammatory phenotype, providing a valuable model to investigate the role of mitochondrial dysfunction in ageing and inflammatory pathologies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|