AMOT130/YAP pathway in topography-induced BMSC osteoblastic differentiation

Autor: Lei He, Wenqing Hou, Mengjie Lu, Lu Xiaobo, Fangping Han, Tailin Guo, Jie Weng, Xuan Liu, Ke Duan
Rok vydání: 2019
Předmět:
Surface Properties
Cell
Osteocalcin
Primary Cell Culture
Cell Culture Techniques
Core Binding Factor Alpha 1 Subunit
02 engineering and technology
01 natural sciences
Mechanotransduction
Cellular
Rats
Sprague-Dawley
Colloid and Surface Chemistry
Osteogenesis
0103 physical sciences
Gene expression
medicine
Animals
Physical and Theoretical Chemistry
Mechanotransduction
Titanium
Nanotubes
Osteoblasts
010304 chemical physics
Chemistry
Mesenchymal stem cell
Membrane Proteins
Translation (biology)
Cell Differentiation
Mesenchymal Stem Cells
YAP-Signaling Proteins
Surfaces and Interfaces
General Medicine
021001 nanoscience & nanotechnology
Alkaline Phosphatase
Actins
Cell biology
Rats
medicine.anatomical_structure
Angiomotins
Gene Expression Regulation
Intercellular Signaling Peptides and Proteins
Osteopontin
Bone marrow
Nuclear transport
0210 nano-technology
Apoptosis Regulatory Proteins
Nuclear localization sequence
Biotechnology
Zdroj: Colloids and surfaces. B, Biointerfaces. 182
ISSN: 1873-4367
Popis: Micro/nano-topography (MNT) is an important variable affecting osseointegration of bone biomaterials, but the underlying mechanisms are not fully understood. We probed the role of a AMOT130/YAP pathway in osteoblastic differentiation of bone marrow mesenchymal stems cultured on titanium (Ti) carrying MNTs. Ti surfaces with two well-defined MNTs (TiO2 nanotubes of different diameters and wall thicknesses) were prepared by anodization. Rat BMSCs were cultured on flat Ti and Ti surfaces carrying MNTs, and cell behaviors (i.e., morphology, F-actin development, osteoblastic differentiation, YAP localization) were studied. Ti surfaces carrying MNTs increased F-actin formation, osteoblastic gene expression, and protein AMOT130 production in BMSCs (all vs. flat Ti), and the surface carrying larger nantubes was more effective, confirming osteoblastic differentiation induced by MNTs. Elevation of the AMOT130 level (by inhibiting its degradation) increased the osteoblastic gene expression, F-actin formation, and nuclear localization of YAP. These show that, AMOT130/YAP is an important pathway mediating the translation of MNT signals to BMSC osteoblastic commitment, likely via the cascade: AMOT130 promotion of F-actin formation, increased YAP nuclear import, and activation of osteoblastic gene expression.
Databáze: OpenAIRE
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