| Autor: |
Yvonne Yuling Hu, Chen-Yuan Dong, Keng Chi Cho, Shean-Jen Chen, Chris Xu, Chia Yuan Chang, Hung Wei Su, Wei Chung Yen, Li Chung Cheng |
| Rok vydání: |
2014 |
| Předmět: |
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| Zdroj: |
Biomedical Optics Express. 5:1768 |
| ISSN: |
2156-7085 |
| DOI: |
10.1364/boe.5.001768 |
| Popis: |
Temporal profile distortions reduce excitation efficiency and image quality in temporal focusing-based multiphoton microscopy. In order to compensate the distortions, a wavefront sensorless adaptive optics system (AOS) was integrated into the microscope. The feedback control signal of the AOS was acquired from local image intensity maximization via a hill-climbing algorithm. The control signal was then utilized to drive a deformable mirror in such a way as to eliminate the distortions. With the AOS correction, not only is the axial excitation symmetrically refocused, but the axial resolution with full two-photon excited fluorescence (TPEF) intensity is also maintained. Hence, the contrast of the TPEF image of a R6G-doped PMMA thin film is enhanced along with a 3.7-fold increase in intensity. Furthermore, the TPEF image quality of 1μm fluorescent beads sealed in agarose gel at different depths is improved. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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