A sandwich-type electrochemical immunosensor for CYFRA 21–1 based on probe-confined in PtPd/polydopamine/hollow carbon spheres coupled with dendritic Au@Rh nanocrystals
Autor: | Yi-Ge Feng, Jia-Wen He, Di-Nan Chen, Lu-Yao Jiang, Ai-Jun Wang, Ning Bao, Jiu-Ju Feng |
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Rok vydání: | 2022 |
Předmět: | |
Zdroj: | Microchimica Acta. 189 |
ISSN: | 1436-5073 0026-3672 |
Popis: | A signal-on sandwich-like electrochemical immunosensor was built for determination of cytokeratin 19 fragments 21-1 (CYFRA 21-1) in non-small cell lung cancer (NSCLC) by confining electroactive dye (e.g., methylene blue, MB) as a probe for amplifying signals. Specifically, core-shell gold@rhodium dendritic nanocrystals (Au@Rh DNCs) behaved as a substrate for primary antibody and accelerate interfacial electron transfer. Besides, hollow carbon spheres (HCSs) were subsequently modified with polydopamine (PDA) and PtPd nanoparticles for sequential integration of the secondary antibody and confinement of MB as a label, termed as MB/PtPd/PDA/HCSs for clarity. The built sensors showed a broad linear range (100 fg mL |
Databáze: | OpenAIRE |
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