A sandwich-type electrochemical immunosensor for CYFRA 21–1 based on probe-confined in PtPd/polydopamine/hollow carbon spheres coupled with dendritic Au@Rh nanocrystals

Autor: Yi-Ge Feng, Jia-Wen He, Di-Nan Chen, Lu-Yao Jiang, Ai-Jun Wang, Ning Bao, Jiu-Ju Feng
Rok vydání: 2022
Předmět:
Zdroj: Microchimica Acta. 189
ISSN: 1436-5073
0026-3672
Popis: A signal-on sandwich-like electrochemical immunosensor was built for determination of cytokeratin 19 fragments 21-1 (CYFRA 21-1) in non-small cell lung cancer (NSCLC) by confining electroactive dye (e.g., methylene blue, MB) as a probe for amplifying signals. Specifically, core-shell gold@rhodium dendritic nanocrystals (Au@Rh DNCs) behaved as a substrate for primary antibody and accelerate interfacial electron transfer. Besides, hollow carbon spheres (HCSs) were subsequently modified with polydopamine (PDA) and PtPd nanoparticles for sequential integration of the secondary antibody and confinement of MB as a label, termed as MB/PtPd/PDA/HCSs for clarity. The built sensors showed a broad linear range (100 fg mL
Databáze: OpenAIRE
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