Evaluation of autophagy-related genes in Egyptian systemic lupus erythematosus patients
| Autor: | Wegdan A. Mohamed, Ayat M. Kamel, Mohamed S. Badary, Ghada Hassan Ahmed, Mohamed A El-Feky |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Anemia Single-nucleotide polymorphism Disease Polymorphism Single Nucleotide Risk Assessment Autophagy-Related Protein 5 03 medical and health sciences 0302 clinical medicine Rheumatology Polymorphism (computer science) Risk Factors Medicine SNP Humans Lupus Erythematosus Systemic Genetic Predisposition to Disease 030212 general & internal medicine RNA Messenger skin and connective tissue diseases Genetic Association Studies 030203 arthritis & rheumatology Complement component 4 Complement component 3 business.industry Interleukin Complement C4 Complement C3 medicine.disease Interleukin-10 Phenotype Case-Control Studies Immunology Beclin-1 Egypt Female business Microtubule-Associated Proteins Biomarkers |
| Zdroj: | International journal of rheumatic diseasesREFERENCES. 23(9) |
| ISSN: | 1756-185X |
| Popis: | Disturbances in autophagy are known to be implicated in autoimmune disorders. Many studies have connected polymorphisms in autophagy-related gene 5 (ATG-5) to systemic lupus erythematosus (SLE). Our aim was the determination of the expression level of ATG-5, Beclin-1 and microtubule-associated protein-light chain 3 (LC-3) in Egyptian SLE patients to investigate the impact of disturbances in autophagy genes on the incidence and progression of the disease. Also, we investigated the incidence of single nucleotide polymorphism (SNP) rs573775 in ATG-5 gene among Egyptian SLE patients. Our results showed that the mean levels of Beclin-1, LC-3 and interleukin (IL)-10 transcripts were significantly higher in SLE patients compared to healthy controls. The previous transcripts were positively correlated with SLE Disease Activity Index (SLEDAI). Beclin-1 and LC-3 transcripts were negatively correlated to complement component 3 (C3) levels. Only LC-3 transcripts were negatively correlated to complement component 4 (C4). The rs573775 SNP of ATG-5 with the variant allele was significantly associated with disease susceptibility, conferring a higher risk of SLE development. This variant allele was more prevalent in patients below 30 years, patients with anemia and in patients with anti-double-stranded DNA (dsDNA), confirming the essential role of ATG-5 polymorphism in the susceptibility of Egyptian patients to SLE. |
| Databáze: | OpenAIRE |
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