Developmental and regional expression pattern of a novel NMDA receptor- like subunit (NMDAR-L) in the rodent brain
| Autor: | Nikolaus J. Sucher, JP Yuan, CL Leclerc, CL Chi, Schahram Akbarian, Stuart A. Lipton, EG Jones, MK Wu, David L. Deitcher, Marc Awobuluyi |
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| Rok vydání: | 1995 |
| Předmět: |
Aging
Cerebellum Xenopus Molecular Sequence Data Thalamus Synaptogenesis Rodentia Biology Polymerase Chain Reaction Receptors N-Methyl-D-Aspartate RNA Complementary Mice Piriform cortex mental disorders medicine Animals Tissue Distribution Amino Acid Sequence RNA Messenger Membrane Glycoproteins Neocortex Base Sequence musculoskeletal neural and ocular physiology General Neuroscience Subiculum Brain Articles Entorhinal cortex Rats medicine.anatomical_structure nervous system Molecular Probes Oocytes Neuroscience Nucleus psychological phenomena and processes |
| Zdroj: | The Journal of Neuroscience. 15:6509-6520 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | A novel NMDA receptor-like (NMDAR-L) cDNA was isolated that contained an open reading frame coding for a predicted polypeptide of 1115 amino acids that shares approximately 27% identity with NMDA receptor subunits. In situ hybridization experiments indicated that NMDAR-L mRNA was expressed in the developing rodent CNS. On postnatal day 1 (P1), NMDAR-L mRNA expression was pronounced in the entorhinal cortex, the subiculum and the thalamus, in layer V of the developing neocortex, in the superior and inferior colliculi, and various regions of the hindbrain, excluding the cerebellum. On P5, NMDAR-L mRNA was expressed in layer V of the neocortex, in the entorhinal cortex, in the subiculum, and in the thalamus. On P14, NMDAR-L mRNA was expressed in layers II-VI of the neocortex, in the entorhinal and piriform cortex, in the subiculum and CA1 field, and in the nucleus of the lateral olfactory tract. In the adult brain, NMDAR-L mRNA was detected predominately in the nucleus of the lateral olfactory tract. Injection of NMDAR-L cRNA into Xenopus oocytes did not lead to the expression of homomeric glutamate-activated channels. However, coinjection of the triple combination of NMDAR-L with NMDAR1 and NMDAR2B cRNAs led to a striking decrease in the current magnitude compared to currents obtained after coexpression of the double combination of NMDAR1 with NMDAR2B. While the function of NMDAR-L remains to be established, its developmental and regional expression pattern suggests that NMDAR-L may influence axonal outgrowth and synaptogenesis during brain development. |
| Databáze: | OpenAIRE |
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