Metabolic Regulation of Gene Transcription
| Autor: | Austin L. Gurney, Mary M. McGrane, Edwards A. Park, Jinsong Liu, Yashomita M. Patel, Richard W. Hanson, Steven E. Nizielski, Summer Savon, Marta Giralt, Deborah R. Crawford |
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| Rok vydání: | 1994 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Response element Medicine (miscellaneous) Mice Transgenic E-box Biology Kidney Gene Expression Regulation Enzymologic Mice Genes jun Sp3 transcription factor Animals Humans Cyclic AMP Response Element-Binding Protein Promoter Regions Genetic Post-transcriptional regulation STAT6 Regulation of gene expression Nutrition and Dietetics Activating Transcription Factor 2 Base Sequence General transcription factor Research Genes fos Promoter DNA Cell biology Liver Phosphoenolpyruvate Carboxykinase (GTP) Transcription Factors |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0022-3166 |
| DOI: | 10.1093/jn/124.suppl_8.1533s |
| Popis: | The impact of nutrients on gene expression has become an area of considerable interest as the number of genes coding for key regulatory proteins in metabolic pathways are studied in detail. This has been greatly aided by a number of new techniques developed to study gene transcription in animals. We will use as an example studies on the regulation of transcription of the gene coding for P-enolpyruvate carboxykinase, a key enzyme in hepatic and renal gluconeogenesis. The promoter for P-enolpyruvate carboxykinase contains a number of regulatory elements within 500 bp of the start-site of gene transcription that are required for the response of the gene to metabolic signals. These elements bind tissue-specific transcription factors in complex patterns of interactions, which result in the coordinate control of P-enolpyruvate carboxykinase gene expression. An analysis of the regulation of transcription of this gene involves the use of a number of techniques ranging from gene transfection into cells in culture to the introduction of chimeric genes containing the P-enolpyruvate carboxykinase promoter into transgenic mice. This review presents a progress report on the current status of research on the nutritional and hormonal regulation of transcription of the P-enolpyruvate carboxykinase gene. |
| Databáze: | OpenAIRE |
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