Oxysophocarpine Ameliorates Carrageenan-induced Inflammatory Pain via Inhibiting Expressions of Prostaglandin E2 and Cytokines in Mice
| Autor: | Yu-Xiang Li, Yang Yang, Yang Niu, Jing Wu, Cheng-Jun Zhao, Juan Du, Hai-Qi Qiao, Ru Zhou, Jian-Qiang Yu, Shao-Ju Jin, Tao Sun, Hong-Ling Wang |
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| Rok vydání: | 2015 |
| Předmět: |
Interleukin-1beta
Pain Pharmaceutical Science Pharmacology Carrageenan Dinoprostone Analytical Chemistry Proinflammatory cytokine Mice chemistry.chemical_compound Alkaloids Drug Discovery Extracellular medicine Animals Edema Prostaglandin E2 Inflammation Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Kinase business.industry Anti-Inflammatory Agents Non-Steroidal Organic Chemistry Real-time polymerase chain reaction Gene Expression Regulation Complementary and alternative medicine chemistry Cyclooxygenase 2 Immunology Cytokines Molecular Medicine Immunohistochemistry Phosphorylation business medicine.drug |
| Zdroj: | Planta Medica. 81:791-797 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-0035-1546153 |
| Popis: | Oxysophocarpine is an alkaloid extracted from Sophora alopecuroides. We investigated the analgesic effect of oxysophocarpine on carrageenan-induced inflammatory pain in mice, in order to explore its possible mechanisms. Mouse ear swelling tests and carrageenan-induced paw edema tests were used to investigate the effects of oxysophocarpine on inflammatory pain in mice. Morphological changes on inflamed paw sections were measured by hematoxylin-eosin staining. The mRNA and protein expression of extracellular signal-regulated kinase, phosphorylation of extracellular signal-regulated kinase 1/2, cyclooxygenase-2, tumor necrosis factor α, interleukin-1 beta, interleukin-6 and prostaglandin E2 were investigated by real-time quantitative polymerase chain reaction, immunohistochemistry, western-blot and enzyme-linked immunosorbent assay. In our results, oxysophocarpine shows a significant anti-inflammatory effect in the mouse ear swelling test. Oxysophocarpine also significantly reduced the paw edema volume and improved mechanical allodynia threshold value on carrageenan-induced inflammatory pain, as well as relieved paw tissues inflammatory damage and reduced the numbers of neutrophils in mice. Oxysophocarpine significantly suppressed over-expression of cyclooxygenase-2, tumor necrosis factor α, interleukin-1 beta, interleukin-6 and prostaglandin E2, and inhibited the over-phosphorylation of extracellular signal-regulated kinase 1/2. Based on these findings we propose that oxysophocarpine attenuates inflammatory pain by suppressing the levels of phosphorylation of extracellular signal-regulated kinase 1/2, cyclooxygenase-2, prostaglandin E2, tumor necrosis factor α, interleukin-1 beta and interleukin-6. |
| Databáze: | OpenAIRE |
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