Expression of neuropeptide Y by glutamatergic stimulation in rat C6 glioma cells
| Autor: | Soo-Kyung Kim, Byung-Kil Choe, Song-woo Shin, Gee-Youn Kwon |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Kainic acid Transcription Genetic Dopamine Glutamic Acid Stimulation Biology Cellular and Molecular Neuroscience chemistry.chemical_compound Glutamatergic Internal medicine mental disorders medicine Tumor Cells Cultured Animals Neuropeptide Y RNA Messenger Pentylenetetrazol Kainic Acid Muscimol Reverse Transcriptase Polymerase Chain Reaction Dopaminergic Cell Biology Glioma Neuropeptide Y receptor humanities Rats Gene Expression Regulation Neoplastic Endocrinology nervous system chemistry NMDA receptor Haloperidol Pentylenetetrazole medicine.drug |
| Zdroj: | Neurochemistry international. 36(1) |
| ISSN: | 0197-0186 |
| Popis: | We have investigated the expression of neuropeptide Y (NPY) in C6 glioma cells after the glutamatergic stimulation by the in situ RT-PCR and immunocytochemical techniques. The expression of NPY mRNA correlated well with immunocytological findings in each series of experiments. NPY protein expression was enhanced by glutamate (1, 10, 50, 100 microM, and 1 mM) dose-dependently, and its expression was slightly increased by N-methyl-D-aspartate (NMDA; 1, 10, 100, 500 microM, and 1 mM) and kainic acid (1, 10, 100, 300 microM, and 1 mM). We pretreated the cells with dopamine, haloperidol, pentylenetetrazol, and muscimol before each stimulation. The pentylenetetrazol and muscimol did not significantly alter the patterns of NPY expression induced by the glutamatergic stimulation. On the other hand, the dopamine and haloperidol pretreatment significantly elevated the levels of NPY expression that were induced by NMDA and kainic acid. Our results indicate that NPY release is closely related to glutamatergic stimulation, and it could be dynamically mediated by GABAergic and dopaminergic costimulation. |
| Databáze: | OpenAIRE |
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